Optimizing chromatographic separation : an experiment using an HPLC simulator

R. A. Shalliker, S. Kayillo, G. R. Dennis

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Optimization of a chromatographic separation within the time constraints of a laboratory session is practically impossible. However, by employing a HPLC simulator, experiments can be designed that allow students to develop an appreciation of the complexities involved in optimization procedures. In the present exercise, a HPLC simulator from JCE Software is used to optimize the separation of a nine-component mixture. The relationship between solvent composition and retention factor is measured to determine the solvent composition for maximized separation. Use of Excel spreadsheets assists in the analysis of the data required for the optimization process. As a result, this exercise demonstrates the basics of the iterative process for optimization of separations and application of spreadsheets to solve relatively complex and tedious repetitive problems.
    Original languageEnglish
    Pages (from-to)1265-1268
    Number of pages4
    JournalJournal of Chemical Education
    Volume85
    Issue number9
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Dive into the research topics of 'Optimizing chromatographic separation : an experiment using an HPLC simulator'. Together they form a unique fingerprint.

    Cite this