Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid

D. Barry Scott; Chris B. Court; Clive W. Ronson; Kieran F. Scott; John M. Watson; Peter R. Schofield; John Shine

doi:10.1007/BF00401991

Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid

D. Barry Scott, Chris B. Court, Clive W. Ronson, Kieran F. Scott, John M. Watson, Peter R. Schofield, John Shine

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17 Citations (Scopus)

Abstract

A Rhizobium trifolii symbiotic plasmid specific gene library was constructed and the physical organisation of regions homologous to nifHDK, nifA and nod genes was determined. These symbiotic gene regions were localised to u 25 kb region on the sym-plasmid, pPN1. In addition four copies of a reiterated sequence were identified on this plasmid, with one copy adjacent to nifH. No rearrangement of these reiterated sequences was observed between R. trifolii bacterial and bacteroid DNA. Analysis of a deletion derivative of pPN1 showed that these sequences were spread over a 110 kb region to the left of nifA.

Original language	English
Pages (from-to)	151-157
Number of pages	7
Journal	Archives of Microbiology
Volume	139
Issue number	2-3
DOIs	https://doi.org/10.1007/BF00401991
Publication status	Published - Oct 1984
Externally published	Yes

Keywords

Nitrogen fixation
Nodulation
Plasmid
Reiterated sequences
Rhizobium trifolii
Symbiosis
Symbiotic genes

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10.1007/BF00401991

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title = "Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid",

abstract = "A Rhizobium trifolii symbiotic plasmid specific gene library was constructed and the physical organisation of regions homologous to nifHDK, nifA and nod genes was determined. These symbiotic gene regions were localised to u 25 kb region on the sym-plasmid, pPN1. In addition four copies of a reiterated sequence were identified on this plasmid, with one copy adjacent to nifH. No rearrangement of these reiterated sequences was observed between R. trifolii bacterial and bacteroid DNA. Analysis of a deletion derivative of pPN1 showed that these sequences were spread over a 110 kb region to the left of nifA.",

keywords = "Nitrogen fixation, Nodulation, Plasmid, Reiterated sequences, Rhizobium trifolii, Symbiosis, Symbiotic genes",

author = "Scott, {D. Barry} and Court, {Chris B.} and Ronson, {Clive W.} and Scott, {Kieran F.} and Watson, {John M.} and Schofield, {Peter R.} and John Shine",

year = "1984",

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doi = "10.1007/BF00401991",

language = "English",

volume = "139",

pages = "151--157",

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T1 - Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid

AU - Scott, D. Barry

AU - Court, Chris B.

AU - Ronson, Clive W.

AU - Scott, Kieran F.

AU - Watson, John M.

AU - Schofield, Peter R.

AU - Shine, John

PY - 1984/10

Y1 - 1984/10

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KW - Nitrogen fixation

KW - Nodulation

KW - Plasmid

KW - Reiterated sequences

KW - Rhizobium trifolii

KW - Symbiosis

KW - Symbiotic genes

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JO - Archives of Microbiology

JF - Archives of Microbiology

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Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid

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Keywords

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