Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae : the involvement of the SWI6 transcription factor

Chii Shyang Fong, Mark D. Temple, Nazif Alic, Joyce Chiu, Moritz Durchdewald, Geoffrey W. Thorpe, Vincent J. Higgins, Ian W. Dawes

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    Cells treated with low doses of linoleic acid hydroperoxide (LoaOOH) exhibit a cell-cycle delay that may provide a mechanism to overcome oxidative stress. Strains sensitive to LoaOOH from the genome-wide deletion collection were screened to identify deletants in which the cell-cycle delay phenotype was reduced. Forty-seven deletants were identified that were unable to mount the normal delay response, implicating the product of the deleted gene in the oxidant-mediated cell-cycle delay of the wild-type. Of these genes, SWI6 was of particular interest due to its role in cell-cycle progression through Start. The swi6 deletant strain was delayed on entry into the cell cycle in the absence of an oxidant, and oxidant addition caused no further delay. Transforming the swi6 deletant with SWI6 on a plasmid restored the G1 arrest in response to LoaOOH, indicating that Swi6p is involved in oxidant sensing leading to cell division delay. Micro-array studies identified genes whose expression in response to LoaOOH depended on SWI6. The screening identified 77 genes that were upregulated in the wild-type strain and concurrently downregulated in the swi6 deletant treated with LoaOOH. These data show that functions such as heat shock response, and glucose transport are involved in the response.
    Original languageEnglish
    Pages (from-to)386-399
    Number of pages14
    JournalFEMS Yeast Research
    Volume8
    Issue number3
    DOIs
    Publication statusPublished - 2008

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