Oxidation of protein and submitochondrial particles by asbestos-derived radicals

N. M. Low, P. L. Nicholson, R. T. Dean

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate that asbestos particles can initiate protein oxidation, especially when provided with co-reactants such as hydrogen peroxide. This can lead to protein fragmentation, as shown with albumin as target. Sub- mitochondrial particles are also oxidised by asbestos-derived radicals, leading to lipid peroxidation. These oxidations parallel the capacity of the particles to hydroxylate benzoate in the presence of hydrogen peroxide, but in some cases can be initiated by the particles alone. The surface availability of metals influences the extent of reaction of the different particles, and the removal of metals from the particles by acid washing decreases their reactivity in these systems. Such reactions may be important in the toxicity of asbestos.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalPathogenesis
Volume1
Issue number3
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Asbestos
  • Lipid oxidation
  • Metals
  • Protein oxidation

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