TY - JOUR
T1 - Parallel segmented flow chromatography columns with multiplexed detection : an illustration using antioxidant screening of natural products
AU - Camenzuli, M.
AU - Ritchie, H. J.
AU - Dennis, G. R.
AU - Shalliker, R. A.
PY - 2013
Y1 - 2013
N2 - The combination of high performance liquid chromatography with the diphenylpicrylhydrazyl radical (DPPH ̇) assay, known as the online DPPH ̇ assay, is widely used for the detection of antioxidants in natural products. The key component in the online DPPH ̇ assay is a reaction coil between the chromatography column and the UV detector. This coil is where the antioxidants leaving the column mix and react with DPPH ̇ reagent. The degree to which the antioxidants decolorise the DPPH ̇ reagent is measured spectrophotometrically. Reaction coils are usually larger than 100. μL, which is incompatible with high efficiency columns, especially for compounds with low retention eluting from short columns. In this study we use the online DPPH ̇ assay with active flow technology chromatography columns, a new column format current in development. This column uses a new type of end fitting which allows the flow from the column to be segmented into multiple portions. Flow segmentation reduces the volumetric flow from the column without increasing the analysis time. The multiple outlet ports on the active flow column enabled detectors to be multiplexed in parallel, rather than in series, providing faster analysis with less dead volume, and greater sensitivity.
AB - The combination of high performance liquid chromatography with the diphenylpicrylhydrazyl radical (DPPH ̇) assay, known as the online DPPH ̇ assay, is widely used for the detection of antioxidants in natural products. The key component in the online DPPH ̇ assay is a reaction coil between the chromatography column and the UV detector. This coil is where the antioxidants leaving the column mix and react with DPPH ̇ reagent. The degree to which the antioxidants decolorise the DPPH ̇ reagent is measured spectrophotometrically. Reaction coils are usually larger than 100. μL, which is incompatible with high efficiency columns, especially for compounds with low retention eluting from short columns. In this study we use the online DPPH ̇ assay with active flow technology chromatography columns, a new column format current in development. This column uses a new type of end fitting which allows the flow from the column to be segmented into multiple portions. Flow segmentation reduces the volumetric flow from the column without increasing the analysis time. The multiple outlet ports on the active flow column enabled detectors to be multiplexed in parallel, rather than in series, providing faster analysis with less dead volume, and greater sensitivity.
UR - http://handle.uws.edu.au:8081/1959.7/533781
U2 - 10.1016/j.microc.2013.07.014
DO - 10.1016/j.microc.2013.07.014
M3 - Article
SN - 0026-265X
VL - 110
SP - 726
EP - 730
JO - Microchemical Journal
JF - Microchemical Journal
ER -