TY - JOUR
T1 - Parallel segmented outlet flow high performance liquid chromatography with multiplexed detection
AU - Camenzuli, Michelle
AU - Terry, Jessica M.
AU - Shalliker, R. Andrew
AU - Conlan, Xavier A.
AU - Barnett, Neil W.
AU - Francis, Paul S.
PY - 2013
Y1 - 2013
N2 - We describe a new approach to multiplex detection for HPLC, exploiting parallel segmented outlet flow - a new column technology that provides pressure-regulated control of eluate flow through multiple outlet channels, which minimises the additional dead volume associated with conventional post-column flow splitting. Using three detectors: one UV-absorbance and two chemiluminescence systems (tris(2,2'-bipyridine)ruthenium(III) and permanganate), we examine the relative responses for six opium poppy (Papaver somniferum) alkaloids under conventional and multiplexed conditions, where approximately 30% of the eluate was distributed to each detector and the remaining solution directed to a collection vessel. The parallel segmented outlet flow mode of operation offers advantages in terms of solvent consumption, waste generation, total analysis time and solute band volume when applying multiple detectors to HPLC, but the manner in which each detection system is influenced by changes in solute concentration and solution flow rates must be carefully considered.
AB - We describe a new approach to multiplex detection for HPLC, exploiting parallel segmented outlet flow - a new column technology that provides pressure-regulated control of eluate flow through multiple outlet channels, which minimises the additional dead volume associated with conventional post-column flow splitting. Using three detectors: one UV-absorbance and two chemiluminescence systems (tris(2,2'-bipyridine)ruthenium(III) and permanganate), we examine the relative responses for six opium poppy (Papaver somniferum) alkaloids under conventional and multiplexed conditions, where approximately 30% of the eluate was distributed to each detector and the remaining solution directed to a collection vessel. The parallel segmented outlet flow mode of operation offers advantages in terms of solvent consumption, waste generation, total analysis time and solute band volume when applying multiple detectors to HPLC, but the manner in which each detection system is influenced by changes in solute concentration and solution flow rates must be carefully considered.
UR - http://handle.uws.edu.au:8081/1959.7/536908
U2 - 10.1016/j.aca.2013.04.014
DO - 10.1016/j.aca.2013.04.014
M3 - Article
SN - 0003-2670
VL - 803
SP - 154
EP - 159
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -