Parathyroid hormone receptor 1 (PTHR1) is a prognostic indicator in canine osteosarcoma

Awf A. Al-Khan; Judith S. Nimmo; Mourad Tayebi; Stewart D. Ryan; James O. Simcock; Raboola Tarzi; Charles A. Kuntz; Eman S. Saad; Michael J. Day; Samantha J. Richardson; Janine A. Danks

doi:10.1038/s41598-020-58524-3

Parathyroid hormone receptor 1 (PTHR1) is a prognostic indicator in canine osteosarcoma

Awf A. Al-Khan, Judith S. Nimmo, Mourad Tayebi, Stewart D. Ryan, James O. Simcock, Raboola Tarzi, Charles A. Kuntz, Eman S. Saad, Michael J. Day, Samantha J. Richardson, Janine A. Danks

Western Sydney University

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Osteosarcoma (OS) is the most common malignant primary bone tumour in humans and dogs. Several studies have established the vital role of parathyroid hormone-related protein (PTHrP) and its receptor (PTHR1) in bone formation and remodeling. In addition, these molecules play a role in the progression and metastasis of many human tumour types. This study investigated the expression of PTHR1 and PTHrP in canine OS tissues and assessed their prognostic value. Formalin-fixed, paraffin-embedded tissue samples from 50 dogs diagnosed with primary OS were immunolabeled with antibodies specific for PTHR1 and PTHrP. The immunostaining intensity of tumours from patients with OS was correlated with survival time. Both PTHR1 and PTHrP were detected in all OS samples (n = 50). Dogs with OS tumours showing high immunostaining intensity for PTHR1 (n = 36) had significantly shorter survival times (p = 0.028, Log Rank; p = 0.04, Cox regression) when compared with OS that had low immunostaining intensity for PTHR1 (n = 14).PTHrP immunostaining intensity did not correlate with survival time (p > 0.05). The results of this study indicate that increased expression of PTHR1 antigen in canine OS is associated with poor prognosis. This suggests that PTHR1 may be useful as a prognostic indicator in canine OS.

Original language	English
Article number	1564
Number of pages	10
Journal	Scientific Reports
Volume	10
DOIs	https://doi.org/10.1038/s41598-020-58524-3
Publication status	Published - 2020

Open Access - Access Right Statement

Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Â© The Author(s) 2020

Keywords

dogs
osteosarcoma
parathyroid hormone

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s41598-020-58524-3

Cite this

@article{b29a2d119b864c6da4074a31225dd105,

title = "Parathyroid hormone receptor 1 (PTHR1) is a prognostic indicator in canine osteosarcoma",

abstract = "Osteosarcoma (OS) is the most common malignant primary bone tumour in humans and dogs. Several studies have established the vital role of parathyroid hormone-related protein (PTHrP) and its receptor (PTHR1) in bone formation and remodeling. In addition, these molecules play a role in the progression and metastasis of many human tumour types. This study investigated the expression of PTHR1 and PTHrP in canine OS tissues and assessed their prognostic value. Formalin-fixed, paraffin-embedded tissue samples from 50 dogs diagnosed with primary OS were immunolabeled with antibodies specific for PTHR1 and PTHrP. The immunostaining intensity of tumours from patients with OS was correlated with survival time. Both PTHR1 and PTHrP were detected in all OS samples (n = 50). Dogs with OS tumours showing high immunostaining intensity for PTHR1 (n = 36) had significantly shorter survival times (p = 0.028, Log Rank; p = 0.04, Cox regression) when compared with OS that had low immunostaining intensity for PTHR1 (n = 14).PTHrP immunostaining intensity did not correlate with survival time (p > 0.05). The results of this study indicate that increased expression of PTHR1 antigen in canine OS is associated with poor prognosis. This suggests that PTHR1 may be useful as a prognostic indicator in canine OS.",

keywords = "dogs, osteosarcoma, parathyroid hormone",

author = "Al-Khan, {Awf A.} and Nimmo, {Judith S.} and Mourad Tayebi and Ryan, {Stewart D.} and Simcock, {James O.} and Raboola Tarzi and Kuntz, {Charles A.} and Saad, {Eman S.} and Day, {Michael J.} and Richardson, {Samantha J.} and Danks, {Janine A.}",

year = "2020",

doi = "10.1038/s41598-020-58524-3",

language = "English",

volume = "10",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Parathyroid hormone receptor 1 (PTHR1) is a prognostic indicator in canine osteosarcoma

AU - Al-Khan, Awf A.

AU - Nimmo, Judith S.

AU - Tayebi, Mourad

AU - Ryan, Stewart D.

AU - Simcock, James O.

AU - Tarzi, Raboola

AU - Kuntz, Charles A.

AU - Saad, Eman S.

AU - Day, Michael J.

AU - Richardson, Samantha J.

AU - Danks, Janine A.

PY - 2020

Y1 - 2020

N2 - Osteosarcoma (OS) is the most common malignant primary bone tumour in humans and dogs. Several studies have established the vital role of parathyroid hormone-related protein (PTHrP) and its receptor (PTHR1) in bone formation and remodeling. In addition, these molecules play a role in the progression and metastasis of many human tumour types. This study investigated the expression of PTHR1 and PTHrP in canine OS tissues and assessed their prognostic value. Formalin-fixed, paraffin-embedded tissue samples from 50 dogs diagnosed with primary OS were immunolabeled with antibodies specific for PTHR1 and PTHrP. The immunostaining intensity of tumours from patients with OS was correlated with survival time. Both PTHR1 and PTHrP were detected in all OS samples (n = 50). Dogs with OS tumours showing high immunostaining intensity for PTHR1 (n = 36) had significantly shorter survival times (p = 0.028, Log Rank; p = 0.04, Cox regression) when compared with OS that had low immunostaining intensity for PTHR1 (n = 14).PTHrP immunostaining intensity did not correlate with survival time (p > 0.05). The results of this study indicate that increased expression of PTHR1 antigen in canine OS is associated with poor prognosis. This suggests that PTHR1 may be useful as a prognostic indicator in canine OS.

AB - Osteosarcoma (OS) is the most common malignant primary bone tumour in humans and dogs. Several studies have established the vital role of parathyroid hormone-related protein (PTHrP) and its receptor (PTHR1) in bone formation and remodeling. In addition, these molecules play a role in the progression and metastasis of many human tumour types. This study investigated the expression of PTHR1 and PTHrP in canine OS tissues and assessed their prognostic value. Formalin-fixed, paraffin-embedded tissue samples from 50 dogs diagnosed with primary OS were immunolabeled with antibodies specific for PTHR1 and PTHrP. The immunostaining intensity of tumours from patients with OS was correlated with survival time. Both PTHR1 and PTHrP were detected in all OS samples (n = 50). Dogs with OS tumours showing high immunostaining intensity for PTHR1 (n = 36) had significantly shorter survival times (p = 0.028, Log Rank; p = 0.04, Cox regression) when compared with OS that had low immunostaining intensity for PTHR1 (n = 14).PTHrP immunostaining intensity did not correlate with survival time (p > 0.05). The results of this study indicate that increased expression of PTHR1 antigen in canine OS is associated with poor prognosis. This suggests that PTHR1 may be useful as a prognostic indicator in canine OS.

KW - dogs

KW - osteosarcoma

KW - parathyroid hormone

UR - https://hdl.handle.net/1959.7/uws:55449

U2 - 10.1038/s41598-020-58524-3

DO - 10.1038/s41598-020-58524-3

M3 - Article

SN - 2045-2322

VL - 10

JO - Scientific Reports

JF - Scientific Reports

M1 - 1564

ER -

Parathyroid hormone receptor 1 (PTHR1) is a prognostic indicator in canine osteosarcoma

Abstract

Open Access - Access Right Statement

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this