TY - JOUR
T1 - Performance of columns packed with the new shell particles, Kinetex-C18
AU - Gritti, Fabrice
AU - Leonardis, Irene
AU - Shock, David
AU - Stevenson, Paul
AU - Shalliker, Andrew
AU - Guiochon, Georges
PY - 2010
Y1 - 2010
N2 - The performance of the new Kinetex-C18 column was investigated. Packed with a new brand of porous shell particles, this column has an outstanding efficiency. Once corrected for the contribution of the instrument extra column volume, the minimum values of the reduced plate heights for a number of low molecular weight compounds (e.g., anthracene and naphtho[2,3-a]pyrene) were between 1.0 and 1.3, breaking the legendary record set 3 years ago by Halo-C18 packed columns. The liquid-solid mass transfer of proteins (e.g., insulin and lyzozyme) is exceptionally fast on Kinetex-C18 much faster than on the Halo-C18 column. The different contributions of dispersion and mass transfer resistances to the column efficiency were determined and discussed. The possible reasons for this extremely high column efficiency are discussed.
AB - The performance of the new Kinetex-C18 column was investigated. Packed with a new brand of porous shell particles, this column has an outstanding efficiency. Once corrected for the contribution of the instrument extra column volume, the minimum values of the reduced plate heights for a number of low molecular weight compounds (e.g., anthracene and naphtho[2,3-a]pyrene) were between 1.0 and 1.3, breaking the legendary record set 3 years ago by Halo-C18 packed columns. The liquid-solid mass transfer of proteins (e.g., insulin and lyzozyme) is exceptionally fast on Kinetex-C18 much faster than on the Halo-C18 column. The different contributions of dispersion and mass transfer resistances to the column efficiency were determined and discussed. The possible reasons for this extremely high column efficiency are discussed.
UR - http://handle.uws.edu.au:8081/1959.7/553282
U2 - 10.1016/j.chroma.2009.12.079
DO - 10.1016/j.chroma.2009.12.079
M3 - Article
SN - 0021-9673
VL - 1217
SP - 1589
EP - 1603
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 10
ER -