Phospholipase A2 activity of crocodile serum

Timo J. Nevalainen; Sumolya Kanchanapangka; Panya Youngprapakorn; Grahame J. W. Webb; S. Charlie Manolis; Kieran F. Scott

doi:10.1163/156853809787392676

Phospholipase A2 activity of crocodile serum

Timo J. Nevalainen, Sumolya Kanchanapangka, Panya Youngprapakorn, Grahame J. W. Webb, S. Charlie Manolis, Kieran F. Scott

Western Sydney University

Research output: Contribution to journal › Article › peer-review

Abstract

The catalytic activity of phospholipase A2 (PLA2) was measured in serum samples from 32 crocodiles in Thailand and Australia by a method using 14C-oleic acid-labelled autoclaved Escherichia coli membranes as a substrate. The highest PLA2 activity was measured in the serum of Crocodylus siamensis (n = 9, mean ± SD), 13.3 ± 3.1 U/l followed by hybrid C. siamensis × C. porosus (n = 6), 10.4 ± 8.7 U/l and Crocodylus porosus (n = 17), 4.3 ± 3.0 U/l. The difference between C. siamensis and C. porosus was highly significant (P < 0.001). The gender of the animals and the geographical location of the crocodile farms were not significant variables affecting serum PLA2 levels. It was concluded that PLA2 is present in crocodilian serum and may have an antimicrobial role.

Original language	English
Pages (from-to)	119-125
Number of pages	7
Journal	Amphibia Reptilia
Volume	30
Issue number	1
DOIs	https://doi.org/10.1163/156853809787392676
Publication status	Published - 2009

Keywords

Crocodylus
Crocodylus siamensis
immunity
infection
inflammation
serum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1163/156853809787392676

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title = "Phospholipase A2 activity of crocodile serum",

abstract = "The catalytic activity of phospholipase A2 (PLA2) was measured in serum samples from 32 crocodiles in Thailand and Australia by a method using 14C-oleic acid-labelled autoclaved Escherichia coli membranes as a substrate. The highest PLA2 activity was measured in the serum of Crocodylus siamensis (n = 9, mean ± SD), 13.3 ± 3.1 U/l followed by hybrid C. siamensis × C. porosus (n = 6), 10.4 ± 8.7 U/l and Crocodylus porosus (n = 17), 4.3 ± 3.0 U/l. The difference between C. siamensis and C. porosus was highly significant (P < 0.001). The gender of the animals and the geographical location of the crocodile farms were not significant variables affecting serum PLA2 levels. It was concluded that PLA2 is present in crocodilian serum and may have an antimicrobial role.",

keywords = "Crocodylus, Crocodylus siamensis, immunity, infection, inflammation, serum",

author = "Nevalainen, {Timo J.} and Sumolya Kanchanapangka and Panya Youngprapakorn and Webb, {Grahame J. W.} and Manolis, {S. Charlie} and Scott, {Kieran F.}",

year = "2009",

doi = "10.1163/156853809787392676",

language = "English",

volume = "30",

pages = "119--125",

journal = "Amphibia Reptilia",

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TY - JOUR

T1 - Phospholipase A2 activity of crocodile serum

AU - Nevalainen, Timo J.

AU - Kanchanapangka, Sumolya

AU - Youngprapakorn, Panya

AU - Webb, Grahame J. W.

AU - Manolis, S. Charlie

AU - Scott, Kieran F.

PY - 2009

Y1 - 2009

N2 - The catalytic activity of phospholipase A2 (PLA2) was measured in serum samples from 32 crocodiles in Thailand and Australia by a method using 14C-oleic acid-labelled autoclaved Escherichia coli membranes as a substrate. The highest PLA2 activity was measured in the serum of Crocodylus siamensis (n = 9, mean ± SD), 13.3 ± 3.1 U/l followed by hybrid C. siamensis × C. porosus (n = 6), 10.4 ± 8.7 U/l and Crocodylus porosus (n = 17), 4.3 ± 3.0 U/l. The difference between C. siamensis and C. porosus was highly significant (P < 0.001). The gender of the animals and the geographical location of the crocodile farms were not significant variables affecting serum PLA2 levels. It was concluded that PLA2 is present in crocodilian serum and may have an antimicrobial role.

AB - The catalytic activity of phospholipase A2 (PLA2) was measured in serum samples from 32 crocodiles in Thailand and Australia by a method using 14C-oleic acid-labelled autoclaved Escherichia coli membranes as a substrate. The highest PLA2 activity was measured in the serum of Crocodylus siamensis (n = 9, mean ± SD), 13.3 ± 3.1 U/l followed by hybrid C. siamensis × C. porosus (n = 6), 10.4 ± 8.7 U/l and Crocodylus porosus (n = 17), 4.3 ± 3.0 U/l. The difference between C. siamensis and C. porosus was highly significant (P < 0.001). The gender of the animals and the geographical location of the crocodile farms were not significant variables affecting serum PLA2 levels. It was concluded that PLA2 is present in crocodilian serum and may have an antimicrobial role.

KW - Crocodylus

KW - Crocodylus siamensis

KW - immunity

KW - infection

KW - inflammation

KW - serum

UR - https://hdl.handle.net/1959.7/uws:56776

U2 - 10.1163/156853809787392676

DO - 10.1163/156853809787392676

M3 - Article

SN - 0173-5373

VL - 30

SP - 119

EP - 125

JO - Amphibia Reptilia

JF - Amphibia Reptilia

IS - 1

ER -

Phospholipase A2 activity of crocodile serum

Abstract

Keywords

UN SDGs

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