Photodynamic treatment of human breast and prostate cancer cells using rose bengal-encapsulated nanoparticles

Mir Muhammad Nasir Uddin; Alina Bekmukhametova; Anu Antony; Shital K. Barman; Jessica Houang; Ming J. Wu; James Hook; Laurel George; Richard Wuhrer; Damia Mawad; Daniel Ta; Antonio Lauto

doi:10.3390/molecules28196901

Photodynamic treatment of human breast and prostate cancer cells using rose bengal-encapsulated nanoparticles

Mir Muhammad Nasir Uddin, Alina Bekmukhametova, Anu Antony, Shital K. Barman, Jessica Houang, Ming J. Wu, James Hook, Laurel George, Richard Wuhrer, Damia Mawad, Daniel Ta, Antonio Lauto

Western Sydney University

Research output: Contribution to journal › Article › peer-review

Abstract

Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily nternalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm2, and irradiance ~0.21 W/cm2). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.

Original language	English
Article number	6901
Number of pages	19
Journal	Molecules
Volume	28
Issue number	19
DOIs	https://doi.org/10.3390/molecules28196901
Publication status	Published - Oct 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Open Access - Access Right Statement

©2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CCBY) license (https://creativecommons.org/licenses/by/4.0/).

Notes

WIP in RD

Keywords

lasers
tumors
reactive oxygen species

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/molecules28196901

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title = "Photodynamic treatment of human breast and prostate cancer cells using rose bengal-encapsulated nanoparticles",

abstract = "Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily nternalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm2, and irradiance ~0.21 W/cm2). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.",

keywords = "lasers, tumors, reactive oxygen species",

author = "Uddin, {Mir Muhammad Nasir} and Alina Bekmukhametova and Anu Antony and Barman, {Shital K.} and Jessica Houang and Wu, {Ming J.} and James Hook and Laurel George and Richard Wuhrer and Damia Mawad and Daniel Ta and Antonio Lauto",

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doi = "10.3390/molecules28196901",

language = "English",

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T1 - Photodynamic treatment of human breast and prostate cancer cells using rose bengal-encapsulated nanoparticles

AU - Uddin, Mir Muhammad Nasir

AU - Bekmukhametova, Alina

AU - Antony, Anu

AU - Barman, Shital K.

AU - Houang, Jessica

AU - Wu, Ming J.

AU - Hook, James

AU - George, Laurel

AU - Wuhrer, Richard

AU - Mawad, Damia

AU - Ta, Daniel

AU - Lauto, Antonio

PY - 2023/10

Y1 - 2023/10

N2 - Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily nternalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm2, and irradiance ~0.21 W/cm2). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.

AB - Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily nternalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm2, and irradiance ~0.21 W/cm2). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.

KW - lasers

KW - tumors

KW - reactive oxygen species

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DO - 10.3390/molecules28196901

M3 - Article

SN - 1420-3049

VL - 28

JO - Molecules

JF - Molecules

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ER -

Photodynamic treatment of human breast and prostate cancer cells using rose bengal-encapsulated nanoparticles

Abstract

Bibliographical note

Open Access - Access Right Statement

Notes

Keywords

UN SDGs

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