Pitfalls in the use of arachidonic acid oxidation products to assign lipoxygenase activity in cancer cells

Marzieh Niknami; Qihan Dong; Paul K. Witting

doi:10.1080/10715760903145013

Pitfalls in the use of arachidonic acid oxidation products to assign lipoxygenase activity in cancer cells

Marzieh Niknami, Qihan Dong, Paul K. Witting

Research output: Contribution to journal › Article › peer-review

Abstract

Arachidonic acid (AA) reaction with cyclooxygenase (COX) and lipoxygenases (LOX) yield eicosanoids that can mediate prostate cancer proliferation and enhance both tumour vascularization and metastasis. Increasingly measurement of eicosanoids with liquid chromatography is employed to implicate LOX activity in different biological systems and in particular link LOX activity to the progression of cancer in experimental models. This study demonstrates that simply identifying patterns of eicosanoid regio-isomerism is insufficient to designate LOX activity in prostate cancer cells and the analysis must include complete stereochemical assignment of the various isomers in order to validate the assignment of LOX activity.

Original language	English
Pages (from-to)	951-956
Number of pages	6
Journal	Free Radical Research
Volume	43
Issue number	10
DOIs	https://doi.org/10.1080/10715760903145013
Publication status	Published - 2009

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10.1080/10715760903145013

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abstract = "Arachidonic acid (AA) reaction with cyclooxygenase (COX) and lipoxygenases (LOX) yield eicosanoids that can mediate prostate cancer proliferation and enhance both tumour vascularization and metastasis. Increasingly measurement of eicosanoids with liquid chromatography is employed to implicate LOX activity in different biological systems and in particular link LOX activity to the progression of cancer in experimental models. This study demonstrates that simply identifying patterns of eicosanoid regio-isomerism is insufficient to designate LOX activity in prostate cancer cells and the analysis must include complete stereochemical assignment of the various isomers in order to validate the assignment of LOX activity.",

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AU - Niknami, Marzieh

AU - Dong, Qihan

AU - Witting, Paul K.

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Y1 - 2009

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AB - Arachidonic acid (AA) reaction with cyclooxygenase (COX) and lipoxygenases (LOX) yield eicosanoids that can mediate prostate cancer proliferation and enhance both tumour vascularization and metastasis. Increasingly measurement of eicosanoids with liquid chromatography is employed to implicate LOX activity in different biological systems and in particular link LOX activity to the progression of cancer in experimental models. This study demonstrates that simply identifying patterns of eicosanoid regio-isomerism is insufficient to designate LOX activity in prostate cancer cells and the analysis must include complete stereochemical assignment of the various isomers in order to validate the assignment of LOX activity.

UR - http://handle.uws.edu.au:8081/1959.7/553440

UR - http://informahealthcare.com/doi/pdf/10.1080/10715760903145013

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EP - 956

JO - Free Radical Research

JF - Free Radical Research

IS - 10

ER -

Pitfalls in the use of arachidonic acid oxidation products to assign lipoxygenase activity in cancer cells

Abstract

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