Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia

Sebastiaan J. van Hal; Bjorn A. Espedido; Geoffrey W. Coombs; Benjamin P. Howden; Tony M. Korman; Graeme R. Nimmo; Iain B. Gosbell; Slade O. Jensen

Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia

Sebastiaan J. van Hal, Bjorn A. Espedido, Geoffrey W. Coombs, Benjamin P. Howden, Tony M. Korman, Graeme R. Nimmo, Iain B. Gosbell, Slade O. Jensen

Western Sydney University

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associatedwith the dissemination of a single ‘dominant’ ST or vanA-encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.

Original language	English
Pages (from-to)	998-1001
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	72
Issue number	4
Publication status	Published - 2017

Bibliographical note

Publisher Copyright:
© The Author 2016.

Keywords

enterococcal infections
genomes
vancomycin
vancomycin resistance

Cite this

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title = "Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia",

abstract = "Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associatedwith the dissemination of a single {\textquoteleft}dominant{\textquoteright} ST or vanA-encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.",

keywords = "enterococcal infections, genomes, vancomycin, vancomycin resistance",

author = "Hal, {Sebastiaan J. van} and Espedido, {Bjorn A.} and Coombs, {Geoffrey W.} and Howden, {Benjamin P.} and Korman, {Tony M.} and Nimmo, {Graeme R.} and Gosbell, {Iain B.} and Jensen, {Slade O.}",

note = "Publisher Copyright: {\textcopyright} The Author 2016.",

year = "2017",

language = "English",

volume = "72",

pages = "998--1001",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "1460-2091",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia

AU - Hal, Sebastiaan J. van

AU - Espedido, Bjorn A.

AU - Coombs, Geoffrey W.

AU - Howden, Benjamin P.

AU - Korman, Tony M.

AU - Nimmo, Graeme R.

AU - Gosbell, Iain B.

AU - Jensen, Slade O.

N1 - Publisher Copyright: © The Author 2016.

PY - 2017

Y1 - 2017

N2 - Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associatedwith the dissemination of a single ‘dominant’ ST or vanA-encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.

AB - Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associatedwith the dissemination of a single ‘dominant’ ST or vanA-encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.

KW - enterococcal infections

KW - genomes

KW - vancomycin

KW - vancomycin resistance

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:38391

M3 - Article

SN - 1460-2091

SN - 0305-7453

VL - 72

SP - 998

EP - 1001

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 4

ER -

Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia

Abstract

Bibliographical note

Keywords

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