TY - JOUR
T1 - Protocol for the visualisation of axial temperature gradients in ultra high performance liquid chromatography using infrared cameras
AU - Vera, C. M.
AU - Samuelsson, J.
AU - Fornstedt, T.
AU - Dennis, G. R.
AU - Shalliker, R. A.
PY - 2018
Y1 - 2018
N2 - A protocol was developed for the visualisation of axial temperature gradients on a Kinetex column (1.3 μm C18 100 Ã
50 x 2.1 mm) operated at near maximum pressure of the system (Pmax) using an infrared camera. Real time viscous frictional heating effects across the entire column length was observed, and showed that with increasing flow rate there was an increase in the maximum temperature of the column, and the difference between the inlet and outlet temperatures. Temperature profile data over the entire length of the column revealed the dynamics of heat exchange processes along different parts of the column, and raises the question on potential heating effects on eluents. The axial temperature gradients of eluents such as pure methanol, isopropyl alcohol and acetonitrile near Pmax were compared; finding that acetonitrile which had the highest flow velocity at Pmax gave the highest overall temperature increase for these eluents.
AB - A protocol was developed for the visualisation of axial temperature gradients on a Kinetex column (1.3 μm C18 100 Ã
50 x 2.1 mm) operated at near maximum pressure of the system (Pmax) using an infrared camera. Real time viscous frictional heating effects across the entire column length was observed, and showed that with increasing flow rate there was an increase in the maximum temperature of the column, and the difference between the inlet and outlet temperatures. Temperature profile data over the entire length of the column revealed the dynamics of heat exchange processes along different parts of the column, and raises the question on potential heating effects on eluents. The axial temperature gradients of eluents such as pure methanol, isopropyl alcohol and acetonitrile near Pmax were compared; finding that acetonitrile which had the highest flow velocity at Pmax gave the highest overall temperature increase for these eluents.
UR - https://hdl.handle.net/1959.7/uws:64854
U2 - 10.1016/j.microc.2018.05.004
DO - 10.1016/j.microc.2018.05.004
M3 - Article
VL - 141
SP - 141
EP - 147
JO - Microchemical Journal
JF - Microchemical Journal
ER -