Pulsed gradient spin-echo NMR studies of the interactions of platinum complexes

Pulsed gradient spin-echo (PGSE) nuclear magnetic resonance (NMR) spectroscopy is increasingly popular among chemists and biochemists. PGSE NMR is non-invasive and is also sensitive to concentrations and diffusion coefficients as low as ,0.1 mM and 10-15m2 s-1, respectively. It has been used to probe aggregation, ion pairing, and reaction mechanisms of metal complexes. The simplest application of PGSE NMR is for the calculation of rS values and the detection of aggregation of PCs in solution. Measurements of 1H and 31P NMR signals of several PCs using PGSE NMR showed that both ligand geometry and molecular weight affect the rS values of PCs. PGSE NMR has been used sparingly for characterizing interactions between PCs and biomolecules. PGSE NMR may provide novel insights into the subtle interactions of these complexes with DNA considering the differences in the n and average apparent association constant values determined for bovine serum albumin (BSA)-PC interactions.