@inproceedings{aa15408d1e8d42dcb9f0f1c0bb9d533a,
title = "Purification of miRNA from whole blood by chemical lysis and phase separation in a centrifugo-pneumatic micro-homogenizer",
abstract = "In this paper a method for the efficient chemical extraction of miRNA from whole blood is first reported. Our novel, cen-trifugo-pneumatic homogenization scheme allows fast mixing of whole blood with the chemical TRI Reagent{\textregistered} (TRI) to provide a very efficient cellular lysis and later separation of the blood homogenate. Thus, at the end of the process, the sample is separated in two phases, an aqueous and an organic phase, with the total RNA fraction remaining in the aqueous phase separated from the DNA and proteins entrapped in the organic phase. To avoid chemical degradation of the chip, a solvent resistant cyclo olefin polymer (Zeonor{\textregistered}) disc was used in this case.",
keywords = "Centrifugal microfluidics, Microhomogenizer, MiRNA extraction, Whole blood",
author = "Linares, {Ana V.} and Robert Gorkin and Barry Glynn and Neus Godino and Nicola Miller and Michael Kerin and Thomas Barry and Terry Smith and Jens Ducr{\'e}e",
year = "2011",
language = "English",
isbn = "9781618395955",
series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
pages = "1460--1462",
booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",
}