Quadruplex DNA-stabilising dinuclear platinum(II) terpyridine complexes with flexible linkers

Dale L. Ang; Benjamin W. J. Harper; Leticia Cubo; Oscar Mendoza; Ramon Vilar; Janice Aldrich-Wright

doi:10.1002/chem.201503663

Quadruplex DNA-stabilising dinuclear platinum(II) terpyridine complexes with flexible linkers

Dale L. Ang, Benjamin W. J. Harper, Leticia Cubo, Oscar Mendoza, Ramon Vilar, Janice Aldrich-Wright

Western Sydney University

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Four dinuclear terpyridineplatinum(II) (Pt–terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 Â°C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt–terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.

Original language	English
Pages (from-to)	2317-2325
Number of pages	9
Journal	Chemistry: A European Journal
Volume	22
Issue number	7
DOIs	https://doi.org/10.1002/chem.201503663
Publication status	Published - 2016

Keywords

DNA
circular dichroism
platinum
quadruplex nucleic acids

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10.1002/chem.201503663

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title = "Quadruplex DNA-stabilising dinuclear platinum(II) terpyridine complexes with flexible linkers",

abstract = "Four dinuclear terpyridineplatinum(II) (Pt–terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 {\^A}°C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt–terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.",

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author = "Ang, {Dale L.} and Harper, {Benjamin W. J.} and Leticia Cubo and Oscar Mendoza and Ramon Vilar and Janice Aldrich-Wright",

year = "2016",

doi = "10.1002/chem.201503663",

language = "English",

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TY - JOUR

T1 - Quadruplex DNA-stabilising dinuclear platinum(II) terpyridine complexes with flexible linkers

AU - Ang, Dale L.

AU - Harper, Benjamin W. J.

AU - Cubo, Leticia

AU - Mendoza, Oscar

AU - Vilar, Ramon

AU - Aldrich-Wright, Janice

PY - 2016

Y1 - 2016

N2 - Four dinuclear terpyridineplatinum(II) (Pt–terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 Â°C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt–terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.

AB - Four dinuclear terpyridineplatinum(II) (Pt–terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 Â°C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt–terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.

KW - DNA

KW - circular dichroism

KW - platinum

KW - quadruplex nucleic acids

UR - http://handle.uws.edu.au:8081/1959.7/uws:33992

U2 - 10.1002/chem.201503663

DO - 10.1002/chem.201503663

M3 - Article

SN - 0947-6539

VL - 22

SP - 2317

EP - 2325

JO - Chemistry: A European Journal

JF - Chemistry: A European Journal

IS - 7

ER -

Quadruplex DNA-stabilising dinuclear platinum(II) terpyridine complexes with flexible linkers

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