Abstract
The subcellular distribution of β-glucuronidase and other hydrolases in rabbit liver was investigated. β-Glucuronidase was found in both microsomal and lysosomal fractions. 2. Multiple forms of β-glucuronidase were present in extracts of microsomal and lysosomal fractions. All forms were common to both fractions. 3. A specific antiserum against β-glucuronidase was raised, and characterized by immunoprecipitation and affinity-chromatography procedures. 4. The immunological identity of the multiple forms in the pure β-glucuronidase preparation, and the immunological identity of the β-glucuronidase complement of lysosomal extracts with that of microsomal extracts, were demonstrated by means of the antiserum. The presence of inactive enzyme in various enzyme preparations was shown.
| Original language | English |
|---|---|
| Pages (from-to) | 407–413 |
| Number of pages | 7 |
| Journal | The Biochemical Journal |
| Volume | 138 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1974 |
| Externally published | Yes |