Radial sample load distribution under overload conditions : analytical scale columns

Neil N. D. Sharma, Gary R. Dennis, R. Andrew Shalliker

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1 Citation (Scopus)

Abstract

The homogeneity of the sample load across the radial cross section of analytical scale columns was determined when operating under overload conditions. The study was performed using active flow technology columns operating in parallel segmentation mode. The outlet segmentation ratio was varied to enable different volume fractions of mobile phase, and thus sample, to elute from the peripheral and central flow regions of the column. The amount of solute exiting the peripheral and radial central exit ports was determined as a function of the flow segmentation ratio. The experimental data using an analytical scale column with dimensions, 100 x 4.6 mm, indicated that the sample load distribution was essentially uniform as a function of the column radial cross section.
Original languageEnglish
Pages (from-to)978-983
Number of pages6
JournalJournal of Liquid Chromatography and Related Technologies
Volume40
Issue number19
DOIs
Publication statusPublished - 2017

Keywords

  • active flow technology
  • chromatography

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