Rapid separation of all four tocopherol homologues in selected fruit seeds via supercritical fluid chromatography using a solid-core C18 column

Elise Urvaka; Inga Misina; Arianne Soliven; Pawel Gornas

doi:10.1155/2019/5307340

Rapid separation of all four tocopherol homologues in selected fruit seeds via supercritical fluid chromatography using a solid-core C18 column

Elise Urvaka, Inga Misina, Arianne Soliven, Pawel Gornas

Western Sydney University

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Tocopherol separations employing the same Kinetexâ„¢ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2"‰min. The RP-LC approach could not separate the isomers Î² and Î³. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.

Original language	English
Article number	5307340
Number of pages	10
Journal	Journal of Chemistry
Volume	2019
DOIs	https://doi.org/10.1155/2019/5307340
Publication status	Published - 2019

Open Access - Access Right Statement

Copyright © 2019 Elise Urvaka et al. This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

fruit
liquid chromatography
seeds
supercritical fluid chromatography
vitamin E

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10.1155/2019/5307340

Cite this

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abstract = "Tocopherol separations employing the same Kinetex{\^a}„¢ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2{"}‰min. The RP-LC approach could not separate the isomers {\^I}² and {\^I}³. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.",

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T1 - Rapid separation of all four tocopherol homologues in selected fruit seeds via supercritical fluid chromatography using a solid-core C18 column

AU - Urvaka, Elise

AU - Misina, Inga

AU - Soliven, Arianne

AU - Gornas, Pawel

PY - 2019

Y1 - 2019

N2 - Tocopherol separations employing the same Kinetexâ„¢ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2"‰min. The RP-LC approach could not separate the isomers Î² and Î³. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.

AB - Tocopherol separations employing the same Kinetexâ„¢ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2"‰min. The RP-LC approach could not separate the isomers Î² and Î³. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.

KW - fruit

KW - liquid chromatography

KW - seeds

KW - supercritical fluid chromatography

KW - vitamin E

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:51035

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DO - 10.1155/2019/5307340

M3 - Article

SN - 2090-9063

VL - 2019

JO - Journal of Chemistry

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M1 - 5307340

ER -

Rapid separation of all four tocopherol homologues in selected fruit seeds via supercritical fluid chromatography using a solid-core C18 column

Abstract

Open Access - Access Right Statement

Keywords

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