Abstract
There have to date no advances in column technology that are able to provide the benefits of increased sensitivity, efficiency, and speed in a single design format. Usually, gains in sensitivity come at the cost of speed, since separations need to be run at a flow rate near the minimum in a HETP curve. Curtain flow chromatography, and even PSF chromatography, overcomes this limitation. Likewise, the cost of column efficiency is usually paid for in the currency of time. But AFT columns can provide higher efficiency than conventional columns, especially as the flow rate increases. Hence, the AFT columns function at their best when separations are run fast. Another factor that usually makes the cost of speed too much to bear is the limitation in detection processing, especially if the detector is a mass spectrometer. With AFT columns, less solvent enters the MS, in fact, in direct proportion to the segmentation ratio. Thus, ultrafast, highly efficient, and very sensitive assays can be undertaken using AFT columns with MS detectors.
Original language | English |
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Title of host publication | Analytical Separation Science |
Editors | Jared L. Anderson, Alain Berthod, Veronica Pino Estevez, Apryll M. Stalcup |
Place of Publication | Germany |
Publisher | Wiley-VCH |
Pages | 43-62 |
Number of pages | 20 |
ISBN (Print) | 9783527333745 |
Publication status | Published - 2015 |
Keywords
- separation (technology)