Reporting guidelines for terrestrial respirometry: building openness, transparency of metabolic rate and evaporative water loss data

Nicholas C. Wu, Lesley Alton, Rafael P. Bovo, Nicholas Carey, Shannon E. Currie, John R.B. Lighton, Andrew E. McKechnie, Patrice Pottier, Giulia Rossi, Craig R. White, Danielle L. Levesque

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Abstract

Respirometry is an important tool for understanding whole-animal energy and water balance in relation to the environment. Consequently, the growing number of studies using respirometry over the last decade warrants reliable reporting and data sharing for effective dissemination and research synthesis. We provide a checklist guideline on five key sections to facilitate the transparency, reproducibility, and replicability of respirometry studies: 1) materials, set up, plumbing, 2) subject conditions/maintenance, 3) measurement conditions, 4) data processing, and 5) data reporting and statistics, each with explanations and example studies. Transparency in reporting and data availability has benefits on multiple fronts. Authors can use this checklist to design and report on their study, and reviewers and editors can use the checklist to assess the reporting quality of the manuscripts they review. Improved standards for reporting will enhance the value of primary studies and will greatly facilitate the ability to carry out higher quality research syntheses to address ecological and evolutionary theories.
Original languageEnglish
Article number111688
Number of pages12
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume296
Issue numberPart A
DOIs
Publication statusPublished - Oct 2024

Bibliographical note

Publisher Copyright:
© 2023

Keywords

  • Comparative physiology
  • Energy metabolism
  • Flow-through
  • Indirect calorimetry
  • Open science
  • Research design

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