Responses of respiration in the light to warming in field-grown trees : a comparison of the thermal sensitivity of the Kok and Laisk methods

Danielle A. Way, Michael J. Aspinwall, John E. Drake, Kristine Y. Crous, Courtney E. Campany, Oula Ghannoum, David T. Tissue, Mark G. Tjoelker

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

The Kok and Laisk techniques can both be used to estimate light respiration R light . We investigated whether responses of R light to short- and long-term changes in leaf temperature depend on the technique used to estimate R light. We grew Eucalyptus tereticornis in whole-tree chambers under ambient temperature (AT) or AT + 3 ° C (elevated temperature, ET). We assessed dark respiration R dark and light respiration with the Kok ( R Kok ) and Laisk ( R Laisk ) methods at four temperatures to determine the degree of light suppression of respiration using both methods in AT and ET trees. The ET treatment had little impact on R dark , R Kok or R Laisk. Although the thermal sensitivities of R Kok or R Laisk were similar, R Kok was higher than R Laisk. We found negative values of R Laisk at the lowest measurement temperatures, indicating positive net CO 2 uptake, which we pro- pose may be related to phosphoenolpyruvate carboxylase activity. Light suppression of R dark decreased with increasing leaf temperature, but the degree of suppression depended on the method used. The Kok and Laisk methods do not generate the same estimates of R light or light suppression of R dark between 20 and 35 ° C. Negative rates of R Laisk imply that this method may become less reliable at low temperatures.
Original languageEnglish
Pages (from-to)132-143
Number of pages12
JournalNew Phytologist
Volume222
Issue number1
DOIs
Publication statusPublished - 2019

Keywords

  • Eucalyptus tereticornis
  • acclimatization
  • carbon cycle (biogeochemistry)
  • climatic changes
  • global warming
  • respiration
  • temperature

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