Revealing the mobilization and age of estuarine dissolved organic matter during floods using radiocarbon and molecular fingerprints

Estuaries significantly affect the transport of dissolved organic matter (DOM) from land to ocean. While the transport and composition of estuarine DOM have been extensively studied, the direct link between DOM chemistry and its age remains unclear, limiting a comprehensive understanding of the dynamics and fate of estuarine DOM under severe conditions (e.g., floods). This study applied radiocarbon and ultrahigh-resolution mass spectrometry analysis to investigate the correlation between DOM chemistry and apparent radiocarbon age of 102 samples collected from the Yangtze River Estuary during both non-flood and flood periods. The results showed that young estuarine DOM are characterized by low-molecular-weight, unsaturated molecules, while aged estuarine DOM are relatively saturated with high-molecular-weight molecules. Phosphorus and nitrogen-containing compounds were key to DOM aging, potentially increasing the lability of aged DOM. Floods significantly impact DOM by introducing more labile aged DOM and young terrestrial DOM. Furthermore, floods enhanced the flux of aged DOM transported to the East China Sea by approximately 1.4 times. Our findings contribute to the study of estuarine DOM and its response during severe floods. Additionally, incorporating apparent radiocarbon age evidence improves the understanding of terrigenous DOM and its fate in large river estuaries before it contributes to the ocean carbon reservoir.