Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

Sai Wang Seto; Ho Yeung Lam; Wing Sze Lau; Alice L. S. Au; Tsz Yan Lam; Stephen S. C. Chim; Sai Ming Ngai; Shun Wan Chan; Tak Yeung Leung; John H. K. Yeung; Siu Kai Kong; George P. H. Leung; Simon M. Y. Lee; Yiu Wa Kwan

doi:10.1016/j.ejphar.2008.12.054

Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

Sai Wang Seto, Ho Yeung Lam, Wing Sze Lau, Alice L. S. Au, Tsz Yan Lam, Stephen S. C. Chim, Sai Ming Ngai, Shun Wan Chan, Tak Yeung Leung, John H. K. Yeung, Siu Kai Kong, George P. H. Leung, Simon M. Y. Lee, Yiu Wa Kwan

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Abstract

We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (NÏ‰-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.

Original language	English
Pages (from-to)	129-137
Number of pages	9
Journal	European Journal of Pharmacology
Volume	605
Issue number	45352
DOIs	https://doi.org/10.1016/j.ejphar.2008.12.054
Publication status	Published - 2009

Keywords

monoamine oxidase
preeclampsia
serotonin
umbilical artery

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10.1016/j.ejphar.2008.12.054

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Seto, S. W., Lam, H. Y., Lau, W. S., Au, A. L. S., Lam, T. Y., Chim, S. S. C., Ngai, S. M., Chan, S. W., Leung, T. Y., Yeung, J. H. K., Kong, S. K., Leung, G. P. H., Lee, S. M. Y., & Kwan, Y. W. (2009). Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery. European Journal of Pharmacology, 605(45352), 129-137. https://doi.org/10.1016/j.ejphar.2008.12.054

@article{31704ca48f3745c4aeb36b820a3a946f,

title = "Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery",

abstract = "We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (N{\"I}‰-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.",

keywords = "monoamine oxidase, preeclampsia, serotonin, umbilical artery",

author = "Seto, {Sai Wang} and Lam, {Ho Yeung} and Lau, {Wing Sze} and Au, {Alice L. S.} and Lam, {Tsz Yan} and Chim, {Stephen S. C.} and Ngai, {Sai Ming} and Chan, {Shun Wan} and Leung, {Tak Yeung} and Yeung, {John H. K.} and Kong, {Siu Kai} and Leung, {George P. H.} and Lee, {Simon M. Y.} and Kwan, {Yiu Wa}",

year = "2009",

doi = "10.1016/j.ejphar.2008.12.054",

language = "English",

volume = "605",

pages = "129--137",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier B.V.",

number = "45352",

}

Seto, SW, Lam, HY, Lau, WS, Au, ALS, Lam, TY, Chim, SSC, Ngai, SM, Chan, SW, Leung, TY, Yeung, JHK, Kong, SK, Leung, GPH, Lee, SMY & Kwan, YW 2009, 'Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery', European Journal of Pharmacology, vol. 605, no. 45352, pp. 129-137. https://doi.org/10.1016/j.ejphar.2008.12.054

TY - JOUR

T1 - Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

AU - Seto, Sai Wang

AU - Lam, Ho Yeung

AU - Lau, Wing Sze

AU - Au, Alice L. S.

AU - Lam, Tsz Yan

AU - Chim, Stephen S. C.

AU - Ngai, Sai Ming

AU - Chan, Shun Wan

AU - Leung, Tak Yeung

AU - Yeung, John H. K.

AU - Kong, Siu Kai

AU - Leung, George P. H.

AU - Lee, Simon M. Y.

AU - Kwan, Yiu Wa

PY - 2009

Y1 - 2009

N2 - We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (NÏ‰-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.

AB - We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (NÏ‰-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.

KW - monoamine oxidase

KW - preeclampsia

KW - serotonin

KW - umbilical artery

UR - http://handle.uws.edu.au:8081/1959.7/uws:30465

U2 - 10.1016/j.ejphar.2008.12.054

DO - 10.1016/j.ejphar.2008.12.054

M3 - Article

SN - 0014-2999

VL - 605

SP - 129

EP - 137

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 45352

ER -

Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

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