TY - JOUR
T1 - Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development
AU - Cazzonelli, Christopher I.
AU - Vanstraelen, Marleen
AU - Simon, Sibu
AU - Yin, Kuide
AU - Carron-Arthur, Ashley
AU - Nisar, Nazia
AU - Tarle, Gauri
AU - Cuttriss, Abby J.
AU - Searle, Iain R.
AU - Benkova, Eva
AU - Mathesius, Ulrike
AU - Masle, Josette
AU - Friml, JìřÃ
AU - Pogson, Barry J.
PY - 2013
Y1 - 2013
N2 - Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.
AB - Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.
UR - http://handle.uws.edu.au:8081/1959.7/540830
U2 - 10.1371/journal.pone.0070069
DO - 10.1371/journal.pone.0070069
M3 - Article
C2 - 23922907
SN - 1932-6203
VL - 8
JO - PLoS One
JF - PLoS One
IS - 7
M1 - e70069
ER -