Roundup Ready® soybean gene concentrations in field soil aggregate size classes

David J. Levy-Booth; Robert H. Gulden; Rachel G. Campbell; Jeff R. Powell; John N. Klironomos; K. Peter Pauls; Clarence J. Swanton; Jack T. Trevors; Kari E. Dunfield

doi:10.1111/j.1574-6968.2008.01449.x

Roundup Ready® soybean gene concentrations in field soil aggregate size classes

David J. Levy-Booth, Robert H. Gulden, Rachel G. Campbell, Jeff R. Powell, John N. Klironomos, K. Peter Pauls, Clarence J. Swanton, Jack T. Trevors, Kari E. Dunfield

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5 Citations (Scopus)

Abstract

Roundup ReadyÂ® (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of genetic transformation of soil bacteria. This study used real-time PCR to examine the concentration of cp4 epsps in four field soil aggregate size classes (>2000 μm, 2000–500 μm, 500–250 μm and < 2000 μm fraction contained between 66.62% and 99.18% of total gene copies, although it only accounted for about 30.00% of the sampled soil. Aggregate formation may facilitate persistence of recombinant DNA.

Original language	English
Number of pages	5
Journal	FEMS Microbiology Letters
DOIs	https://doi.org/10.1111/j.1574-6968.2008.01449.x
Publication status	Published - 2009

Keywords

DNA
aggregates
genetically modified
persistance
real-time PCR
soils

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10.1111/j.1574-6968.2008.01449.x

Cite this

@article{b490771de55d46cf9f0b97a819d04c89,

title = "Roundup Ready{\textregistered} soybean gene concentrations in field soil aggregate size classes",

abstract = "Roundup Ready{\^A}{\textregistered} (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of genetic transformation of soil bacteria. This study used real-time PCR to examine the concentration of cp4 epsps in four field soil aggregate size classes (>2000 μm, 2000–500 μm, 500–250 μm and < 2000 μm fraction contained between 66.62% and 99.18% of total gene copies, although it only accounted for about 30.00% of the sampled soil. Aggregate formation may facilitate persistence of recombinant DNA.",

keywords = "DNA, aggregates, genetically modified, persistance, real-time PCR, soils",

author = "Levy-Booth, {David J.} and Gulden, {Robert H.} and Campbell, {Rachel G.} and Powell, {Jeff R.} and Klironomos, {John N.} and Pauls, {K. Peter} and Swanton, {Clarence J.} and Trevors, {Jack T.} and Dunfield, {Kari E.}",

year = "2009",

doi = "10.1111/j.1574-6968.2008.01449.x",

language = "English",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

}

TY - JOUR

T1 - Roundup Ready® soybean gene concentrations in field soil aggregate size classes

AU - Levy-Booth, David J.

AU - Gulden, Robert H.

AU - Campbell, Rachel G.

AU - Powell, Jeff R.

AU - Klironomos, John N.

AU - Pauls, K. Peter

AU - Swanton, Clarence J.

AU - Trevors, Jack T.

AU - Dunfield, Kari E.

PY - 2009

Y1 - 2009

N2 - Roundup ReadyÂ® (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of genetic transformation of soil bacteria. This study used real-time PCR to examine the concentration of cp4 epsps in four field soil aggregate size classes (>2000 μm, 2000–500 μm, 500–250 μm and < 2000 μm fraction contained between 66.62% and 99.18% of total gene copies, although it only accounted for about 30.00% of the sampled soil. Aggregate formation may facilitate persistence of recombinant DNA.

AB - Roundup ReadyÂ® (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of genetic transformation of soil bacteria. This study used real-time PCR to examine the concentration of cp4 epsps in four field soil aggregate size classes (>2000 μm, 2000–500 μm, 500–250 μm and < 2000 μm fraction contained between 66.62% and 99.18% of total gene copies, although it only accounted for about 30.00% of the sampled soil. Aggregate formation may facilitate persistence of recombinant DNA.

KW - DNA

KW - aggregates

KW - genetically modified

KW - persistance

KW - real-time PCR

KW - soils

UR - http://handle.uws.edu.au:8081/1959.7/511765

U2 - 10.1111/j.1574-6968.2008.01449.x

DO - 10.1111/j.1574-6968.2008.01449.x

M3 - Article

SN - 0378-1097

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

ER -

Roundup Ready® soybean gene concentrations in field soil aggregate size classes

Abstract

Keywords

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