Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation

Meenu Mehta; Keshav Raj Paudel; Shakti D. Shukla; Madhur D. Shastri; Saurabh Satija; Sachin Kumar Singh; Monica Gulati; Harish Dureja; Flavia C. Zacconi; Philip M. Hansbro; Dinesh Kumar Chellappan; Kamal Dua

doi:10.4155/fmc-2020-0297

Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation

Meenu Mehta
, Keshav Raj Paudel
, Shakti D. Shukla
, Madhur D. Shastri
, Saurabh Satija
, Sachin Kumar Singh
, Monica Gulati
, Harish Dureja
, Flavia C. Zacconi
, Philip M. Hansbro
, Dinesh Kumar Chellappan
, Kamal Dua

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); ?-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.

Original language	English
Pages (from-to)	543-549
Number of pages	7
Journal	Future Medicinal Chemistry
Volume	13
Issue number	6
DOIs	https://doi.org/10.4155/fmc-2020-0297
Publication status	Published - Mar 2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

antioxidant
inflammation
lipopolysaccharide
oxidative stress genes
rutin

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10.4155/fmc-2020-0297

Cite this

Mehta, M., Paudel, K. R., Shukla, S. D., Shastri, M. D., Satija, S., Singh, S. K., Gulati, M., Dureja, H., Zacconi, F. C., Hansbro, P. M., Chellappan, D. K., & Dua, K. (2021). Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation. Future Medicinal Chemistry, 13(6), 543-549. https://doi.org/10.4155/fmc-2020-0297

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title = "Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation",

abstract = "Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); ?-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.",

keywords = "antioxidant, inflammation, lipopolysaccharide, oxidative stress genes, rutin",

author = "Meenu Mehta and Paudel, \{Keshav Raj\} and Shukla, \{Shakti D.\} and Shastri, \{Madhur D.\} and Saurabh Satija and Singh, \{Sachin Kumar\} and Monica Gulati and Harish Dureja and Zacconi, \{Flavia C.\} and Hansbro, \{Philip M.\} and Chellappan, \{Dinesh Kumar\} and Kamal Dua",

year = "2021",

month = mar,

doi = "10.4155/fmc-2020-0297",

language = "English",

volume = "13",

pages = "543--549",

journal = "Future Medicinal Chemistry",

issn = "1756-8919",

publisher = "Taylor \& Francis",

number = "6",

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TY - JOUR

T1 - Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells

T2 - a PCR validation

AU - Mehta, Meenu

AU - Paudel, Keshav Raj

AU - Shukla, Shakti D.

AU - Shastri, Madhur D.

AU - Satija, Saurabh

AU - Singh, Sachin Kumar

AU - Gulati, Monica

AU - Dureja, Harish

AU - Zacconi, Flavia C.

AU - Hansbro, Philip M.

AU - Chellappan, Dinesh Kumar

AU - Dua, Kamal

PY - 2021/3

Y1 - 2021/3

N2 - Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); ?-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.

AB - Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); ?-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.

KW - antioxidant

KW - inflammation

KW - lipopolysaccharide

KW - oxidative stress genes

KW - rutin

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UR - https://go.openathens.net/redirector/westernsydney.edu.au?url=https://doi.org/10.4155/fmc-2020-0297

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DO - 10.4155/fmc-2020-0297

M3 - Article

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AN - SCOPUS:85102584150

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VL - 13

SP - 543

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JO - Future Medicinal Chemistry

JF - Future Medicinal Chemistry

IS - 6

ER -

Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation

Abstract

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Keywords

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