TY - JOUR
T1 - Secretion by mononuclear phagocytes of lysosomal hydrolases bearing ligands for the mannose-6-phosphate receptor system of fibroblasts
T2 - Evidence for a second mechanism of spontaneous secretion?
AU - Jessup, Wendy
AU - Dean, Roger T.
PY - 1982/4/14
Y1 - 1982/4/14
N2 - β-Hexosaminidase secreted by peritoneal macrophages in response to stimulation by zymosan or NH4Cl, or spontaneously by a macrophage-like cell line (P388D1), is susceptible to receptor-mediated endocytosis by human fibroblasts. This endocytosis is almost completely blocked by exogenous mannose-6-phosphate and therefore seems to depend on a mannose-6-phosphate ligand on the enzyme. It is suggested that macrophage lysosomal enzyme packaging may involve mannose-6-phosphate recognition markers, and that a continuous hypersecretion mechanism may exist which does not depend on a defect in this ligand.
AB - β-Hexosaminidase secreted by peritoneal macrophages in response to stimulation by zymosan or NH4Cl, or spontaneously by a macrophage-like cell line (P388D1), is susceptible to receptor-mediated endocytosis by human fibroblasts. This endocytosis is almost completely blocked by exogenous mannose-6-phosphate and therefore seems to depend on a mannose-6-phosphate ligand on the enzyme. It is suggested that macrophage lysosomal enzyme packaging may involve mannose-6-phosphate recognition markers, and that a continuous hypersecretion mechanism may exist which does not depend on a defect in this ligand.
UR - http://www.scopus.com/inward/record.url?scp=0020377181&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(82)91058-0
DO - 10.1016/0006-291X(82)91058-0
M3 - Article
C2 - 6284168
AN - SCOPUS:0020377181
SN - 0006-291X
VL - 105
SP - 922
EP - 927
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -