Separation of ifosfamide and its degradation products using micellar electrokinetic chromatography

Ifosfamide and its thermal degradation products were separated using micellar electrokinetic chromatography (MEKC). The method was compared with a published reversed phase HPLC method. Much better resolution was obtained using the MEKC method. Nine degradation products could be detected using MEKC compared with only two using reversed phase HPLC. The LOD using the equipment described was 0.05 mg ml^-1 with UV detection at 185 mm. The standard error of the peak area for five injections was 2.8%. The standard error of peak position of the degradation products varied from less than 1% up to 5% with the latter eluting peaks having the higher variation.