Sequence differences in the diagnostic region of the cysteine protease 8 gene of Tritrichomonas foetus parasites of cats and cattle

Zichen Sun; Colin Stack; Jan Slapeta

doi:10.1016/j.vetpar.2011.12.001

Sequence differences in the diagnostic region of the cysteine protease 8 gene of Tritrichomonas foetus parasites of cats and cattle

Zichen Sun, Colin Stack, Jan Slapeta

School of Science

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

In order to investigate the genetic variation between Tritrichomonas foetus from bovine and feline origins, cysteine protease 8 (CP8) coding sequence was selected as the polymorphic DNA marker. Direct sequencing of CP8 coding sequence of T. foetus from four feline isolates and two bovine isolates with polymerase chain reaction successfully revealed conserved nucleotide polymorphisms between feline and bovine isolates. These results provide useful information for CP8-based molecular differentiation of T. foetus genotypes.

Original language	English
Pages (from-to)	445-449
Number of pages	5
Journal	Veterinary Parasitology
Volume	186
Issue number	45385
DOIs	https://doi.org/10.1016/j.vetpar.2011.12.001
Publication status	Published - 2012

Keywords

Tritrichomonas foetus
amino acid sequence
cats
cattle
cysteine proteases
diarrhea
diseases
epidemiology
genotype
host-parasite interactions

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10.1016/j.vetpar.2011.12.001

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abstract = "In order to investigate the genetic variation between Tritrichomonas foetus from bovine and feline origins, cysteine protease 8 (CP8) coding sequence was selected as the polymorphic DNA marker. Direct sequencing of CP8 coding sequence of T. foetus from four feline isolates and two bovine isolates with polymerase chain reaction successfully revealed conserved nucleotide polymorphisms between feline and bovine isolates. These results provide useful information for CP8-based molecular differentiation of T. foetus genotypes.",

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AU - Sun, Zichen

AU - Stack, Colin

AU - Slapeta, Jan

PY - 2012

Y1 - 2012

N2 - In order to investigate the genetic variation between Tritrichomonas foetus from bovine and feline origins, cysteine protease 8 (CP8) coding sequence was selected as the polymorphic DNA marker. Direct sequencing of CP8 coding sequence of T. foetus from four feline isolates and two bovine isolates with polymerase chain reaction successfully revealed conserved nucleotide polymorphisms between feline and bovine isolates. These results provide useful information for CP8-based molecular differentiation of T. foetus genotypes.

AB - In order to investigate the genetic variation between Tritrichomonas foetus from bovine and feline origins, cysteine protease 8 (CP8) coding sequence was selected as the polymorphic DNA marker. Direct sequencing of CP8 coding sequence of T. foetus from four feline isolates and two bovine isolates with polymerase chain reaction successfully revealed conserved nucleotide polymorphisms between feline and bovine isolates. These results provide useful information for CP8-based molecular differentiation of T. foetus genotypes.

KW - Tritrichomonas foetus

KW - amino acid sequence

KW - cats

KW - cattle

KW - cysteine proteases

KW - diarrhea

KW - diseases

KW - epidemiology

KW - genotype

KW - host-parasite interactions

UR - http://handle.uws.edu.au:8081/1959.7/516927

U2 - 10.1016/j.vetpar.2011.12.001

DO - 10.1016/j.vetpar.2011.12.001

M3 - Article

C2 - 22204891

SN - 0304-4017

VL - 186

SP - 445

EP - 449

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 45385

ER -

Sequence differences in the diagnostic region of the cysteine protease 8 gene of Tritrichomonas foetus parasites of cats and cattle

Abstract

Keywords

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