Abstract
Spectral imaging combines the advantages of both spectroscopy and imaging by acquiring sacral information at every pixel in an image. Given the orientation of this book, we will be mostly concerned with the situation where fluorescence emission spectra are recorded using a specialized fluorescence or laser scanning microscope. However, it is important to realize that the concepts and methods of spectral imaging are applicable to a great range of experimental techniques, from energy-dispersive X-ray spectroscopy (EDS), electron energy loss spectroscopy (EELS), and infrared (IR) spectroscopy to Raman spectroscopy, cathodoluminescence (CL) spectroscopy, scanning tunnelling spectroscopy (STS), and mass spectrometry (MS). Spectral imaging is also referred to as hyperspectral imaging, imaging spectroscopy, or multipixel spectroscopy when the number of wavelengths at which the spectra are recorded is large. For a small number of wavelengths, the multispectral imaging is also used.
| Original language | English |
|---|---|
| Title of host publication | Fluorescence Applications in Biotechnology and Life Sciences |
| Editors | Ewa M. Goldys |
| Place of Publication | U.S. |
| Publisher | Wiley-Blackwell |
| Pages | 117-139 |
| Number of pages | 23 |
| ISBN (Print) | 9780470083703 |
| Publication status | Published - 2009 |