Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR

Francesco Cutrale; Anya Salih; Enrico Gratton

doi:10.1088/2050-6120/1/3/035001

Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR

Francesco Cutrale, Anya Salih, Enrico Gratton

Research output: Contribution to journal › Article › peer-review

Abstract

The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.

Original language	English
Article number	35001
Pages (from-to)	1-10
Number of pages	10
Journal	Methods and Applications in Fluorescence
Volume	1
Issue number	3
DOIs	https://doi.org/10.1088/2050-6120/1/3/035001
Publication status	Published - 2013

Access to Document

10.1088/2050-6120/1/3/035001

Cite this

@article{ef1a9629a5ac4c1a9911f254d79508cb,

title = "Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR",

abstract = "The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.",

author = "Francesco Cutrale and Anya Salih and Enrico Gratton",

year = "2013",

doi = "10.1088/2050-6120/1/3/035001",

language = "English",

volume = "1",

pages = "1--10",

journal = "Methods and Applications in Fluorescence",

issn = "2050-6120",

publisher = "Institute of Physics Publishing",

number = "3",

}

TY - JOUR

T1 - Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR

AU - Cutrale, Francesco

AU - Salih, Anya

AU - Gratton, Enrico

PY - 2013

Y1 - 2013

N2 - The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.

AB - The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein.

UR - http://handle.uws.edu.au:8081/1959.7/531625

U2 - 10.1088/2050-6120/1/3/035001

DO - 10.1088/2050-6120/1/3/035001

M3 - Article

SN - 2050-6120

VL - 1

SP - 1

EP - 10

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

IS - 3

M1 - 35001

ER -

Spectral phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR

Abstract

Access to Document

Other files and links

Fingerprint

Cite this