Spirostanol saponins from the fibrous roots of Ophiopogon japonicus (Thunb.) Ker-Gawl

Three new steroidal saponins, (25R)-ruscogenin-3-yl a-l-rhamnopyranosyl- (1→2)-[β-D-xylopyranosyl-( 1→4)]-β-D-glucopyranoside (1), diosgenin-3-yl 2-O-acetyl-a-l-rhamnopyranosyl-(1→2)-[β-D- xylopyranosyl-( 1→4)]-β-D-glucopyranoside (2), and pennogenin-3-yl 2-O-acetyl-α-L-rhamnopyranosyl-(1→2)-[β-D-xylopyranosyl- (1→4)]-β-D-glucopyranoside (3) were isolated from the fibrous roots of Ophiopogon japonicus (THUNB.) KER-GAWL. Their structures were determined by spectroscopic methods including IR, HR-ESI-MS, and 1D- and 2D-NMR. All of these three steroidal saponins exhibited weak cytotoxicities against Hela and Hep2 cell lines.