Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories

Jerry Zhou; Larissa Belov; Pierre Chapuis; Charles Chan; Nicola Armstrong; Kimberley L. Kaufman; Michael J. Solomon; Stephen J. Clarke; Richard I. Christopherson

doi:10.1016/j.jim.2014.11.001

Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories

Jerry Zhou, Larissa Belov, Pierre Chapuis, Charles Chan, Nicola Armstrong, Kimberley L. Kaufman, Michael J. Solomon, Stephen J. Clarke, Richard I. Christopherson

Research output: Contribution to journal › Article › peer-review

Abstract

Extensive surface profiles of colorectal cancer (CRC) cells and tumor infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies. Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.

Original language	English
Pages (from-to)	59-68
Number of pages	10
Journal	Journal of Immunological Methods
Volume	416
DOIs	https://doi.org/10.1016/j.jim.2014.11.001
Publication status	Published - 2014

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@article{1204ffb87e21426d9a370817d7994253,

title = "Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories",

abstract = "Extensive surface profiles of colorectal cancer (CRC) cells and tumor infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies. Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.",

author = "Jerry Zhou and Larissa Belov and Pierre Chapuis and Charles Chan and Nicola Armstrong and Kaufman, {Kimberley L.} and Solomon, {Michael J.} and Clarke, {Stephen J.} and Christopherson, {Richard I.}",

year = "2014",

doi = "10.1016/j.jim.2014.11.001",

language = "English",

volume = "416",

pages = "59--68",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

}

TY - JOUR

T1 - Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories

AU - Zhou, Jerry

AU - Belov, Larissa

AU - Chapuis, Pierre

AU - Chan, Charles

AU - Armstrong, Nicola

AU - Kaufman, Kimberley L.

AU - Solomon, Michael J.

AU - Clarke, Stephen J.

AU - Christopherson, Richard I.

PY - 2014

Y1 - 2014

N2 - Extensive surface profiles of colorectal cancer (CRC) cells and tumor infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies. Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.

AB - Extensive surface profiles of colorectal cancer (CRC) cells and tumor infiltrating lymphocytes (TIL) have been obtained from 45 surgical resection samples. Live cells were captured on an antibody microarray and stained with fluorescently-labeled antibodies. Minimal panels of 11 CRC antigens (CD13, CD24, CD26, CD49d, CD138, CD166, CA-125, CA19-9, EGFR, Galectin-4 and HLA-DR) and 11 T-cell antigens (CD10, CD11b, CD11c, CD25, CD31, CD95, CD151, CD181, Galectin-4, CA19-9, TSP-1) provide signatures for relapse and survival. Hierarchical clustering of profiles from CRC cells and TIL identified groups of patients for survival, systemic relapse and death. The groups from CRC and TIL profiles for systemic relapse showed 79.2% concordance, enabling prediction of relapse after surgery. The results demonstrate communication between CRC cells and TIL.

UR - http://handle.uws.edu.au:8081/1959.7/565184

U2 - 10.1016/j.jim.2014.11.001

DO - 10.1016/j.jim.2014.11.001

M3 - Article

SN - 0022-1759

VL - 416

SP - 59

EP - 68

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

ER -

Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories

Abstract

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