The action of defined oxygen-centred free radicals on human low-density lipoprotein

S. Bedwell; R. T. Dean; W. Jessup

doi:10.1042/bj2620707

The action of defined oxygen-centred free radicals on human low-density lipoprotein

S. Bedwell, R. T. Dean, W. Jessup

Brunel University London

Research output: Contribution to journal › Article › peer-review

162 Citations (Scopus)

Abstract

The effects of defined oxygen-centred free radicals on human low-density lipoprotein (LDL) structure and receptor affinity are discussed in relation to the mechanisms of cell-mediated oxidative modification of LDL. Both hydroxyl (OH^.) and hydroperoxyl (HO₂^.) radicals caused depletion of endogenous α-tocopherol and formation of hydroperoxides. Superoxide (O₂^.-) radicals produced only very limited oxidation, but could potentiate oxidation stimulated by the addition of Cu²⁺. All these radicals enhanced the net negative charge of intact LDL and induced fragmentation of apolipoprotein B-100 (apo B). OH^. also caused cross-linking of apo B. Radical attack decreased the affinity of LDL for the fibroblast apo B/E receptor, but did not enhance its endocytosis by mouse macrophages.

Original language	English
Pages (from-to)	707-712
Number of pages	6
Journal	The Biochemical Journal
Volume	262
Issue number	3
DOIs	https://doi.org/10.1042/bj2620707
Publication status	Published - 1989
Externally published	Yes

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abstract = "The effects of defined oxygen-centred free radicals on human low-density lipoprotein (LDL) structure and receptor affinity are discussed in relation to the mechanisms of cell-mediated oxidative modification of LDL. Both hydroxyl (OH.) and hydroperoxyl (HO2.) radicals caused depletion of endogenous α-tocopherol and formation of hydroperoxides. Superoxide (O2.-) radicals produced only very limited oxidation, but could potentiate oxidation stimulated by the addition of Cu2+. All these radicals enhanced the net negative charge of intact LDL and induced fragmentation of apolipoprotein B-100 (apo B). OH. also caused cross-linking of apo B. Radical attack decreased the affinity of LDL for the fibroblast apo B/E receptor, but did not enhance its endocytosis by mouse macrophages.",

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AU - Bedwell, S.

AU - Dean, R. T.

AU - Jessup, W.

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N2 - The effects of defined oxygen-centred free radicals on human low-density lipoprotein (LDL) structure and receptor affinity are discussed in relation to the mechanisms of cell-mediated oxidative modification of LDL. Both hydroxyl (OH.) and hydroperoxyl (HO2.) radicals caused depletion of endogenous α-tocopherol and formation of hydroperoxides. Superoxide (O2.-) radicals produced only very limited oxidation, but could potentiate oxidation stimulated by the addition of Cu2+. All these radicals enhanced the net negative charge of intact LDL and induced fragmentation of apolipoprotein B-100 (apo B). OH. also caused cross-linking of apo B. Radical attack decreased the affinity of LDL for the fibroblast apo B/E receptor, but did not enhance its endocytosis by mouse macrophages.

AB - The effects of defined oxygen-centred free radicals on human low-density lipoprotein (LDL) structure and receptor affinity are discussed in relation to the mechanisms of cell-mediated oxidative modification of LDL. Both hydroxyl (OH.) and hydroperoxyl (HO2.) radicals caused depletion of endogenous α-tocopherol and formation of hydroperoxides. Superoxide (O2.-) radicals produced only very limited oxidation, but could potentiate oxidation stimulated by the addition of Cu2+. All these radicals enhanced the net negative charge of intact LDL and induced fragmentation of apolipoprotein B-100 (apo B). OH. also caused cross-linking of apo B. Radical attack decreased the affinity of LDL for the fibroblast apo B/E receptor, but did not enhance its endocytosis by mouse macrophages.

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JO - The Biochemical Journal

JF - The Biochemical Journal

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ER -

The action of defined oxygen-centred free radicals on human low-density lipoprotein

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