TY - JOUR
T1 - The antimicrobial properties of some copper(II) and platinum(II) 1,10-phenanthroline complexes
AU - Ng, Neville S.
AU - Leverett, Peter
AU - Hibbs, David E.
AU - Yang, Qianfan
AU - Bulanadi, Jerikho C.
AU - Wu, Ming Jie
AU - Aldrich-Wright, Janice R.
PY - 2013
Y1 - 2013
N2 - Copper(II) (1(Cu)-21(Cu)) and previously established experimental anticancer platinum(II) metallointercalator complexes (1(Pt)-16(Pt)) have been prepared and investigated for their antimicrobial properties. These complexes are of the general structure [M(I-L)(A(L))](2+) where I-L represents functionalised 1,10-phenanthrolines (1(IL)-10(IL)), and A(L) represents 1,2-diaminoethane, 1S,2S- or 1R,2R-diaminocyclohexane. The structures of synthesised complexes were confirmed using a combination of elemental analysis, UV spectrometry, circular dichroism, H-1 and [H-1-Pt-195]-HMQC NMR, X-ray crystallography, and electrospray ionisation mass spectrometry and where appropriate. Crystallisation attempts yielded single crystals of [Cu(4-methyl-1,10-phenanthroline)(1R,2R-diaminocyclohexane)](ClO4)(2) (4(Cu)), [Cu(5,6- dimethyl-1,10-phenanthroline)(1R,2R-diaminocyclohexane)(H2O)](ClO4)(2)center dot 1.5H(2)O (10(Cu)) and [Cu(5,6-dimethyl-1,10-phenanthroline)(3)]-(ClO4)(2)center dot 5,6-dimethyl-1,10-phenanthroline center dot 2H(2)O (21(Cu)). Growth inhibition of liquid cultures of bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa), and yeast (Saccharomyces cerevisiae) discerned the most antimicrobially potent metal complexes <= 20 mu M, as well as that of their intercalating ligands alone. To further investigate their mode of antimicrobial activity, membrane permeabilisation caused by selected complexes was visualised by means of a cell viability kit under fluorescence microscopy.
AB - Copper(II) (1(Cu)-21(Cu)) and previously established experimental anticancer platinum(II) metallointercalator complexes (1(Pt)-16(Pt)) have been prepared and investigated for their antimicrobial properties. These complexes are of the general structure [M(I-L)(A(L))](2+) where I-L represents functionalised 1,10-phenanthrolines (1(IL)-10(IL)), and A(L) represents 1,2-diaminoethane, 1S,2S- or 1R,2R-diaminocyclohexane. The structures of synthesised complexes were confirmed using a combination of elemental analysis, UV spectrometry, circular dichroism, H-1 and [H-1-Pt-195]-HMQC NMR, X-ray crystallography, and electrospray ionisation mass spectrometry and where appropriate. Crystallisation attempts yielded single crystals of [Cu(4-methyl-1,10-phenanthroline)(1R,2R-diaminocyclohexane)](ClO4)(2) (4(Cu)), [Cu(5,6- dimethyl-1,10-phenanthroline)(1R,2R-diaminocyclohexane)(H2O)](ClO4)(2)center dot 1.5H(2)O (10(Cu)) and [Cu(5,6-dimethyl-1,10-phenanthroline)(3)]-(ClO4)(2)center dot 5,6-dimethyl-1,10-phenanthroline center dot 2H(2)O (21(Cu)). Growth inhibition of liquid cultures of bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa), and yeast (Saccharomyces cerevisiae) discerned the most antimicrobially potent metal complexes <= 20 mu M, as well as that of their intercalating ligands alone. To further investigate their mode of antimicrobial activity, membrane permeabilisation caused by selected complexes was visualised by means of a cell viability kit under fluorescence microscopy.
KW - DNA cleavage
KW - antibacterial
KW - antibiotics
KW - compounds
KW - cytotoxicity
KW - dimethyl sulfoxide
KW - nuclease activity
KW - permeability
UR - http://handle.uws.edu.au:8081/1959.7/523783
U2 - 10.1039/c2dt32392c
DO - 10.1039/c2dt32392c
M3 - Article
SN - 1477-9226
VL - 42
SP - 3196
EP - 3209
JO - Dalton Transactions
JF - Dalton Transactions
IS - 9
ER -