TY - JOUR
T1 - The assessment of π-π selective stationary phases for two-dimensional HPLC analysis of foods : application to the analysis of coffee
AU - Mnatsakanyan, Mariam
AU - Stevenson, Paul G.
AU - Shock, David
AU - Conlan, Xavier A.
AU - Goodie, Tiffany A.
AU - Spencer, Kylie N.
AU - Barnett, Neil W.
AU - Francis, Paul S.
AU - Shalliker, R. Andrew
PY - 2010
Y1 - 2010
N2 - Differences between alkyl, dipole-dipole, hydrogen bonding, and π-π selective surfaces represented by non-resonance and resonance π-stationary phases have been assessed for the separation of 'Ristretto' café espresso by employing 2DHPLC techniques with C18 phase selectivity detection. Geometric approach to factor analysis (GAFA) was used to measure the detected peaks (N), spreading angle (β), correlation, practical peak capacity (np) and percentage usage of the separations space, as an assessment of selectivity differences between regional quadrants of the two-dimensional separation plane. Although all tested systems were correlated to some degree to the C18 dimension, regional measurement of separation divergence revealed that performance of specific systems was better for certain sample components. The results illustrate that because of the complexity of the 'real' sample obtaining a truly orthogonal two-dimensional system for complex samples of natural origin may be practically impossible.
AB - Differences between alkyl, dipole-dipole, hydrogen bonding, and π-π selective surfaces represented by non-resonance and resonance π-stationary phases have been assessed for the separation of 'Ristretto' café espresso by employing 2DHPLC techniques with C18 phase selectivity detection. Geometric approach to factor analysis (GAFA) was used to measure the detected peaks (N), spreading angle (β), correlation, practical peak capacity (np) and percentage usage of the separations space, as an assessment of selectivity differences between regional quadrants of the two-dimensional separation plane. Although all tested systems were correlated to some degree to the C18 dimension, regional measurement of separation divergence revealed that performance of specific systems was better for certain sample components. The results illustrate that because of the complexity of the 'real' sample obtaining a truly orthogonal two-dimensional system for complex samples of natural origin may be practically impossible.
UR - http://handle.uws.edu.au:8081/1959.7/554210
U2 - 10.1016/j.talanta.2010.06.065
DO - 10.1016/j.talanta.2010.06.065
M3 - Article
SN - 0039-9140
VL - 82
SP - 1349
EP - 1357
JO - Talanta
JF - Talanta
IS - 4
ER -