The assessment of π-π selective stationary phases for two-dimensional HPLC analysis of foods : application to the analysis of coffee

Mariam Mnatsakanyan, Paul G. Stevenson, David Shock, Xavier A. Conlan, Tiffany A. Goodie, Kylie N. Spencer, Neil W. Barnett, Paul S. Francis, R. Andrew Shalliker

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    Differences between alkyl, dipole-dipole, hydrogen bonding, and π-π selective surfaces represented by non-resonance and resonance π-stationary phases have been assessed for the separation of 'Ristretto' café espresso by employing 2DHPLC techniques with C18 phase selectivity detection. Geometric approach to factor analysis (GAFA) was used to measure the detected peaks (N), spreading angle (β), correlation, practical peak capacity (np) and percentage usage of the separations space, as an assessment of selectivity differences between regional quadrants of the two-dimensional separation plane. Although all tested systems were correlated to some degree to the C18 dimension, regional measurement of separation divergence revealed that performance of specific systems was better for certain sample components. The results illustrate that because of the complexity of the 'real' sample obtaining a truly orthogonal two-dimensional system for complex samples of natural origin may be practically impossible.
    Original languageEnglish
    Pages (from-to)1349-1357
    Number of pages9
    JournalTalanta
    Volume82
    Issue number4
    DOIs
    Publication statusPublished - 2010

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