The development of the in situ modification of 1st generation analytical scale silica monoliths

Arianne Soliven, Gary R. Dennis, Emily F. Hilder, R. Andrew Shalliker, Paul G. Stevenson

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Analytical scale silica monoliths are commercially limited to three column selectivities (bare silica, C8 and C18). An in situ modification is reported in detail to overcome this barrier and allow for any functionality of choice to be bonded to the silica surface of the monolithic stationary phase support. The modification method was conducted on a commercial bare silica column to bond the C18 moiety to the silica surface through a silylation reaction. The C18 type of stationary phase was chosen, as this is the most commonly bonded functionality for the majority of stationary phases used for high-performance liquid chromatography (HPLC) separations. The C18-modified monolith's performance was compared to a commercial C18 monolithic and a particle packed column of the same analytical scale column dimensions (100 x 4.6 mm). The modified C18 monolith proved to be of high quality with an efficiency of 73,267 N m-1, fast analysis times (operated at flow rates up to 3 mL min-1 using a conventional 400 bar HPLC system) and improved resolution of a set of polar and non-polar substituted aromatics in comparison to a commercial C18 monolith.
    Original languageEnglish
    Pages (from-to)663-671
    Number of pages9
    JournalChromatographia
    Volume77
    Issue number45574
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Dive into the research topics of 'The development of the in situ modification of 1st generation analytical scale silica monoliths'. Together they form a unique fingerprint.

    Cite this