The expression of SEIPIN in the mouse central nervous system

Xiaoyun Liu; Beibei Xie; Yanfei Qi; Ximing Du; Shaoshi Wang; Yumei Zhang; George Paxinos; Hongyuan Yang; Huazheng Liang

doi:10.1007/s00429-015-1151-3

The expression of SEIPIN in the mouse central nervous system

Xiaoyun Liu, Beibei Xie, Yanfei Qi, Ximing Du, Shaoshi Wang, Yumei Zhang, George Paxinos, Hongyuan Yang, Huazheng Liang

Western Sydney University

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.

Original language	English
Pages (from-to)	4111-4127
Number of pages	17
Journal	Brain Structure and Function
Volume	221
Issue number	8
DOIs	https://doi.org/10.1007/s00429-015-1151-3
Publication status	Published - 2016

Keywords

brain
motor cortex
seipinopathy
spinal cord

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10.1007/s00429-015-1151-3

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title = "The expression of SEIPIN in the mouse central nervous system",

abstract = "Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.",

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author = "Xiaoyun Liu and Beibei Xie and Yanfei Qi and Ximing Du and Shaoshi Wang and Yumei Zhang and George Paxinos and Hongyuan Yang and Huazheng Liang",

year = "2016",

doi = "10.1007/s00429-015-1151-3",

language = "English",

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journal = "Brain Structure and Function",

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T1 - The expression of SEIPIN in the mouse central nervous system

AU - Liu, Xiaoyun

AU - Xie, Beibei

AU - Qi, Yanfei

AU - Du, Ximing

AU - Wang, Shaoshi

AU - Zhang, Yumei

AU - Paxinos, George

AU - Yang, Hongyuan

AU - Liang, Huazheng

PY - 2016

Y1 - 2016

N2 - Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.

AB - Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.

KW - brain

KW - motor cortex

KW - seipinopathy

KW - spinal cord

UR - http://handle.uws.edu.au:8081/1959.7/uws:36839

UR - http://link.springer.com/article/10.1007%2Fs00429-015-1151-3

U2 - 10.1007/s00429-015-1151-3

DO - 10.1007/s00429-015-1151-3

M3 - Article

SN - 1863-2653

VL - 221

SP - 4111

EP - 4127

JO - Brain Structure and Function

JF - Brain Structure and Function

IS - 8

ER -

The expression of SEIPIN in the mouse central nervous system

Abstract

Keywords

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