The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity

Letian Chen; Satoshi Hamada; Masayuki Fujiwara; Tingheng Zhu; Nguyen Phuong Thao; Hann Ling Wong; Priti Krishna; Takashi Ueda; Hanae Kaku; Naoto Shibuya; Tsutomu Kawasaki; Ko Shimamoto

doi:10.1016/j.chom.2010.02.008

The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity

Letian Chen, Satoshi Hamada, Masayuki Fujiwara, Tingheng Zhu, Nguyen Phuong Thao, Hann Ling Wong, Priti Krishna, Takashi Ueda, Hanae Kaku, Naoto Shibuya, Tsutomu Kawasaki, Ko Shimamoto

Western Sydney University

Research output: Contribution to journal › Article › peer-review

158 Citations (Scopus)

Abstract

Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs) represents a critical first step of innate defense in plants and animals. However, maturation and transport of PRRs are not well understood. We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER). Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sar1, a small GTPase which regulates ER-to-Golgi trafficking. Further, Hop/Sti1 and Hsp90 are present at the PM in a complex (designated the "defensome") with OsRac1, a plant-specific Rho-type GTPase. Finally, Hop/Sti1 was required for chitin-triggered immunity and resistance to rice blast fungus. Our results suggest that the Hop/Sti1-Hsp90 chaperone complex plays an important and likely conserved role in the maturation and transport of PRRs and may function to link PRRs and Rac/Rop GTPases.

Original language	English
Pages (from-to)	185-196
Number of pages	12
Journal	Cell Host and Microbe
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/j.chom.2010.02.008
Publication status	Published - 2010

Keywords

chitin
heat shock proteins
natural immunity
plant proteins
rice

Access to Document

10.1016/j.chom.2010.02.008

Cite this

@article{897d9eb3d2fa479aa1f5d9344631824a,

title = "The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity",

abstract = "Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs) represents a critical first step of innate defense in plants and animals. However, maturation and transport of PRRs are not well understood. We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER). Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sar1, a small GTPase which regulates ER-to-Golgi trafficking. Further, Hop/Sti1 and Hsp90 are present at the PM in a complex (designated the {"}defensome{"}) with OsRac1, a plant-specific Rho-type GTPase. Finally, Hop/Sti1 was required for chitin-triggered immunity and resistance to rice blast fungus. Our results suggest that the Hop/Sti1-Hsp90 chaperone complex plays an important and likely conserved role in the maturation and transport of PRRs and may function to link PRRs and Rac/Rop GTPases.",

keywords = "chitin, heat shock proteins, natural immunity, plant proteins, rice",

author = "Letian Chen and Satoshi Hamada and Masayuki Fujiwara and Tingheng Zhu and Thao, {Nguyen Phuong} and Wong, {Hann Ling} and Priti Krishna and Takashi Ueda and Hanae Kaku and Naoto Shibuya and Tsutomu Kawasaki and Ko Shimamoto",

year = "2010",

doi = "10.1016/j.chom.2010.02.008",

language = "English",

volume = "7",

pages = "185--196",

journal = "Cell Host and Microbe",

issn = "1931-3128",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity

AU - Chen, Letian

AU - Hamada, Satoshi

AU - Fujiwara, Masayuki

AU - Zhu, Tingheng

AU - Thao, Nguyen Phuong

AU - Wong, Hann Ling

AU - Krishna, Priti

AU - Ueda, Takashi

AU - Kaku, Hanae

AU - Shibuya, Naoto

AU - Kawasaki, Tsutomu

AU - Shimamoto, Ko

PY - 2010

Y1 - 2010

N2 - Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs) represents a critical first step of innate defense in plants and animals. However, maturation and transport of PRRs are not well understood. We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER). Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sar1, a small GTPase which regulates ER-to-Golgi trafficking. Further, Hop/Sti1 and Hsp90 are present at the PM in a complex (designated the "defensome") with OsRac1, a plant-specific Rho-type GTPase. Finally, Hop/Sti1 was required for chitin-triggered immunity and resistance to rice blast fungus. Our results suggest that the Hop/Sti1-Hsp90 chaperone complex plays an important and likely conserved role in the maturation and transport of PRRs and may function to link PRRs and Rac/Rop GTPases.

AB - Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs) represents a critical first step of innate defense in plants and animals. However, maturation and transport of PRRs are not well understood. We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER). Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sar1, a small GTPase which regulates ER-to-Golgi trafficking. Further, Hop/Sti1 and Hsp90 are present at the PM in a complex (designated the "defensome") with OsRac1, a plant-specific Rho-type GTPase. Finally, Hop/Sti1 was required for chitin-triggered immunity and resistance to rice blast fungus. Our results suggest that the Hop/Sti1-Hsp90 chaperone complex plays an important and likely conserved role in the maturation and transport of PRRs and may function to link PRRs and Rac/Rop GTPases.

KW - chitin

KW - heat shock proteins

KW - natural immunity

KW - plant proteins

KW - rice

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:42180

U2 - 10.1016/j.chom.2010.02.008

DO - 10.1016/j.chom.2010.02.008

M3 - Article

SN - 1931-3128

VL - 7

SP - 185

EP - 196

JO - Cell Host and Microbe

JF - Cell Host and Microbe

IS - 3

ER -

The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this