The in vitro effects of interferon-gamma, alone or in combination with amphotericin B, tested against the pathogenic fungi Candida albicans and Aspergillus fumigatus

Moufid El-Khoury; Rogine Ligot; Simon Mahoney; Colin M. Stack; Gabriel G. Perrone; C. Oliver Morton

The in vitro effects of interferon-gamma, alone or in combination with amphotericin B, tested against the pathogenic fungi Candida albicans and Aspergillus fumigatus

Moufid El-Khoury, Rogine Ligot, Simon Mahoney, Colin M. Stack, Gabriel G. Perrone, C. Oliver Morton

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Objective: Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-Î³). Results: In this study we investigated the direct antifungal effects of recombinant human IFN-Î³ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts. It was found that 32 pg/ml of IFN-Î³ exhibited a significant but small antifungal effect on A. fumigatus (p = 0.02), Aspergillus flavus (p = 0.04) and Saccharomyces cerevisiae (p = 0.03), inhibiting growth by 6, 11 and 17% respectively. No significant inhibitory effects were observed in Candida species (p > 0.05 for all species tested) or Cryptococus neoformans (p = 0.98). Short term exposure (3 h) to a combination of amphotericin B (1 Î¼g/ml) and IFN-Î³ (32 pg/ml) increased the effectiveness of amphotericin B against A. fumigatus and S. cerevisiae but not Candida albicans. These data suggest that IFN-Î³ does not possess strong antifungal activity but can enhance the effect of amphotericin B under some testing conditions against Aspergillus species.

Original language	English
Article number	364
Number of pages	6
Journal	BMC Research Notes
Volume	10
Issue number	1
Publication status	Published - 1 Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 The Author(s).

Open Access - Access Right Statement

© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

Keywords

Aspergillosis
amphotericin B
interleukin-18

Cite this

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title = "The in vitro effects of interferon-gamma, alone or in combination with amphotericin B, tested against the pathogenic fungi Candida albicans and Aspergillus fumigatus",

abstract = "Objective: Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-{\^I}³). Results: In this study we investigated the direct antifungal effects of recombinant human IFN-{\^I}³ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts. It was found that 32 pg/ml of IFN-{\^I}³ exhibited a significant but small antifungal effect on A. fumigatus (p = 0.02), Aspergillus flavus (p = 0.04) and Saccharomyces cerevisiae (p = 0.03), inhibiting growth by 6, 11 and 17% respectively. No significant inhibitory effects were observed in Candida species (p > 0.05 for all species tested) or Cryptococus neoformans (p = 0.98). Short term exposure (3 h) to a combination of amphotericin B (1 {\^I}¼g/ml) and IFN-{\^I}³ (32 pg/ml) increased the effectiveness of amphotericin B against A. fumigatus and S. cerevisiae but not Candida albicans. These data suggest that IFN-{\^I}³ does not possess strong antifungal activity but can enhance the effect of amphotericin B under some testing conditions against Aspergillus species.",

keywords = "Aspergillosis, amphotericin B, interleukin-18",

author = "Moufid El-Khoury and Rogine Ligot and Simon Mahoney and Stack, {Colin M.} and Perrone, {Gabriel G.} and Morton, {C. Oliver}",

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AU - El-Khoury, Moufid

AU - Ligot, Rogine

AU - Mahoney, Simon

AU - Stack, Colin M.

AU - Perrone, Gabriel G.

AU - Morton, C. Oliver

PY - 2017/8/1

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N2 - Objective: Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-Î³). Results: In this study we investigated the direct antifungal effects of recombinant human IFN-Î³ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts. It was found that 32 pg/ml of IFN-Î³ exhibited a significant but small antifungal effect on A. fumigatus (p = 0.02), Aspergillus flavus (p = 0.04) and Saccharomyces cerevisiae (p = 0.03), inhibiting growth by 6, 11 and 17% respectively. No significant inhibitory effects were observed in Candida species (p > 0.05 for all species tested) or Cryptococus neoformans (p = 0.98). Short term exposure (3 h) to a combination of amphotericin B (1 Î¼g/ml) and IFN-Î³ (32 pg/ml) increased the effectiveness of amphotericin B against A. fumigatus and S. cerevisiae but not Candida albicans. These data suggest that IFN-Î³ does not possess strong antifungal activity but can enhance the effect of amphotericin B under some testing conditions against Aspergillus species.

AB - Objective: Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-Î³). Results: In this study we investigated the direct antifungal effects of recombinant human IFN-Î³ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts. It was found that 32 pg/ml of IFN-Î³ exhibited a significant but small antifungal effect on A. fumigatus (p = 0.02), Aspergillus flavus (p = 0.04) and Saccharomyces cerevisiae (p = 0.03), inhibiting growth by 6, 11 and 17% respectively. No significant inhibitory effects were observed in Candida species (p > 0.05 for all species tested) or Cryptococus neoformans (p = 0.98). Short term exposure (3 h) to a combination of amphotericin B (1 Î¼g/ml) and IFN-Î³ (32 pg/ml) increased the effectiveness of amphotericin B against A. fumigatus and S. cerevisiae but not Candida albicans. These data suggest that IFN-Î³ does not possess strong antifungal activity but can enhance the effect of amphotericin B under some testing conditions against Aspergillus species.

KW - Aspergillosis

KW - amphotericin B

KW - interleukin-18

UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:42747

M3 - Article

C2 - 28764751

SN - 1756-0500

VL - 10

JO - BMC Research Notes

JF - BMC Research Notes

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ER -

The in vitro effects of interferon-gamma, alone or in combination with amphotericin B, tested against the pathogenic fungi Candida albicans and Aspergillus fumigatus

Abstract

Bibliographical note

Open Access - Access Right Statement

Keywords

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