The liquid chromatographic determination of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) raw herb

Jarryd L. Pearson; Samiuela Lee; Harsha Suresh; Mitchell Low; Marnilar Nang; Swastika Singh; Franklin Lamin; Magdy Kazzem; Shaun Sullivan; Cheang S. Khoo

doi:10.1155/2014/968314

The liquid chromatographic determination of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) raw herb

Jarryd L. Pearson, Samiuela Lee, Harsha Suresh, Mitchell Low, Marnilar Nang, Swastika Singh, Franklin Lamin, Magdy Kazzem, Shaun Sullivan, Cheang S. Khoo

Research output: Contribution to journal › Article › peer-review

Abstract

A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS.AC18 column was usedwith a 0.1% aqueous formic acid : methanol gradientmobile phase. The analytes were quantified 325 nm.With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced.The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63mg/g, respectively, and the method LOD was 0.13 and 0.02mg/g, respectively.

Original language	English
Article number	968314
Number of pages	6
Journal	ISRN Analytical Chemistry
Volume	2014
DOIs	https://doi.org/10.1155/2014/968314
Publication status	Published - 2014

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title = "The liquid chromatographic determination of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) raw herb",

abstract = "A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS.AC18 column was usedwith a 0.1% aqueous formic acid : methanol gradientmobile phase. The analytes were quantified 325 nm.With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced.The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63mg/g, respectively, and the method LOD was 0.13 and 0.02mg/g, respectively.",

author = "Pearson, {Jarryd L.} and Samiuela Lee and Harsha Suresh and Mitchell Low and Marnilar Nang and Swastika Singh and Franklin Lamin and Magdy Kazzem and Shaun Sullivan and Khoo, {Cheang S.}",

year = "2014",

doi = "10.1155/2014/968314",

language = "English",

volume = "2014",

journal = "ISRN Analytical Chemistry",

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TY - JOUR

T1 - The liquid chromatographic determination of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) raw herb

AU - Pearson, Jarryd L.

AU - Lee, Samiuela

AU - Suresh, Harsha

AU - Low, Mitchell

AU - Nang, Marnilar

AU - Singh, Swastika

AU - Lamin, Franklin

AU - Kazzem, Magdy

AU - Sullivan, Shaun

AU - Khoo, Cheang S.

PY - 2014

Y1 - 2014

N2 - A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS.AC18 column was usedwith a 0.1% aqueous formic acid : methanol gradientmobile phase. The analytes were quantified 325 nm.With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced.The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63mg/g, respectively, and the method LOD was 0.13 and 0.02mg/g, respectively.

AB - A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS.AC18 column was usedwith a 0.1% aqueous formic acid : methanol gradientmobile phase. The analytes were quantified 325 nm.With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced.The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63mg/g, respectively, and the method LOD was 0.13 and 0.02mg/g, respectively.

UR - http://handle.uws.edu.au:8081/1959.7/545817

U2 - 10.1155/2014/968314

DO - 10.1155/2014/968314

M3 - Article

SN - 2090-7311

VL - 2014

JO - ISRN Analytical Chemistry

JF - ISRN Analytical Chemistry

M1 - 968314

ER -

The liquid chromatographic determination of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) raw herb

Abstract

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