TY - JOUR
T1 - The liquid chromatographic determination of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) raw herb
AU - Pearson, Jarryd L.
AU - Lee, Samiuela
AU - Suresh, Harsha
AU - Low, Mitchell
AU - Nang, Marnilar
AU - Singh, Swastika
AU - Lamin, Franklin
AU - Kazzem, Magdy
AU - Sullivan, Shaun
AU - Khoo, Cheang S.
PY - 2014
Y1 - 2014
N2 - A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS.AC18 column was usedwith a 0.1% aqueous formic acid : methanol gradientmobile phase. The analytes were quantified 325 nm.With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced.The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63mg/g, respectively, and the method LOD was 0.13 and 0.02mg/g, respectively.
AB - A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS.AC18 column was usedwith a 0.1% aqueous formic acid : methanol gradientmobile phase. The analytes were quantified 325 nm.With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced.The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63mg/g, respectively, and the method LOD was 0.13 and 0.02mg/g, respectively.
UR - http://handle.uws.edu.au:8081/1959.7/545817
U2 - 10.1155/2014/968314
DO - 10.1155/2014/968314
M3 - Article
SN - 2090-7311
VL - 2014
JO - ISRN Analytical Chemistry
JF - ISRN Analytical Chemistry
M1 - 968314
ER -