The performance of second generation silica monoliths, operating in parallel segmented flow mode

This study investigates the chromatographic performance of second generation monoliths in its conventional design, as well as replacing the standard outlet with an Active Flow Technology (AFT) assembly to minimise inefficient flow contributions. The AFT coupled monolith resulted in up to 50% improvement on the conventional monolith's efficiency and a 16% improvement in sensitivity. Exploitation of the current second generation monolith's silica infrastructure and AFT coupling in the parallel segmented flow (PSF) mode of operation would benefit high through-put analyses that demand highly efficient, sensitive, low volume detection, front end separation solutions utilizing conventional HPLC systems.