Time-dependence of aggregation in crystallizing Iysozyme solutions using PGSE-NMR self diffusion measurements

William S. Price, Fumihiko Tsuchiya, Yoji Arata

    Research output: Contribution to journalArticle

    Abstract

    The time dependence of aggregation in supersaturated lysozyme solutions was studied using pulsed-gradient spin-echo NMR diffusion measurements as a function of lysozyme concentration at pH 6.0 and 298 K in the presence of 0.5 M NaCl. The measurements provide estimates of the weight-averaged diffusion coefficient of the monomeric to intermediate molecular weight lysozyme species present in the solution (very large aggregates and crystals are excluded from the average due to the NMR relaxation-weighting effects inherent in the method). The results show that the average molecular weight of the various lysozyme aggregates changed with sigmoidal kinetics and that these kinetics were strongly influenced by the initial lysozyme concentration. The visualization of the time dependence of the protein aggregation afforded by this method provides a deeper understanding of how the crystallizing conditions (especially the initial protein concentration) are related to the resulting crystals.
    Original languageEnglish
    JournalBiophysical Journal
    Publication statusPublished - 2001

    Keywords

    • diffusion measurements
    • lysozyme
    • molecular weights

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