Toward a full characterization of native starch : separation and detection by size-exclusion chromatography

Ngoc-Ly Hoang; Antonin Landolfi; Anastasiya Kravchuk; Etienne Girard; Jonathan Peate; Javier M. Hernandez; Marianne Gaborieau; Olena Kravchuk; Robert G. Gilbert; Yohann Guillaneuf; Patrice Castignolles

Toward a full characterization of native starch : separation and detection by size-exclusion chromatography

Ngoc-Ly Hoang, Antonin Landolfi, Anastasiya Kravchuk, Etienne Girard, Jonathan Peate, Javier M. Hernandez, Marianne Gaborieau, Olena Kravchuk, Robert G. Gilbert, Yohann Guillaneuf, Patrice Castignolles

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44 Citations (Scopus)

Abstract

The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 Ãƒ"šÃ‚Â°C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.

Original language	English
Pages (from-to)	60-70
Number of pages	11
Journal	Journal of Chromatography. A
Volume	1205
Issue number	45323
Publication status	Published - 2008

Keywords

gel permeation chromatography
separation (technology)

Cite this

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author = "Ngoc-Ly Hoang and Antonin Landolfi and Anastasiya Kravchuk and Etienne Girard and Jonathan Peate and Hernandez, {Javier M.} and Marianne Gaborieau and Olena Kravchuk and Gilbert, {Robert G.} and Yohann Guillaneuf and Patrice Castignolles",

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TY - JOUR

T1 - Toward a full characterization of native starch : separation and detection by size-exclusion chromatography

AU - Hoang, Ngoc-Ly

AU - Landolfi, Antonin

AU - Kravchuk, Anastasiya

AU - Girard, Etienne

AU - Peate, Jonathan

AU - Hernandez, Javier M.

AU - Gaborieau, Marianne

AU - Kravchuk, Olena

AU - Gilbert, Robert G.

AU - Guillaneuf, Yohann

AU - Castignolles, Patrice

PY - 2008

Y1 - 2008

N2 - The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 Ãƒ"šÃ‚Â°C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.

AB - The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 Ãƒ"šÃ‚Â°C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.

KW - gel permeation chromatography

KW - separation (technology)

UR - http://handle.uws.edu.au:8081/1959.7/504959

M3 - Article

SN - 0021-9673

VL - 1205

SP - 60

EP - 70

JO - Journal of Chromatography. A

JF - Journal of Chromatography. A

IS - 45323

ER -

Toward a full characterization of native starch : separation and detection by size-exclusion chromatography

Abstract

Keywords

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