Abstract
The susceptibility of procyclic, trypsinized, or bloodstream forms of Trypanosoma brucei to lysis by hydrogen peroxide and by activated mouse macrophages was investigated in vitro using the release of biosynthetically labeled proteins as an assay. Uncoated parasites were more resistant than coated bloodstream forms in both cases. Macrophage trypanolysis upon triggering with phorbol myristate acetate occurred extracellularly and seemed to depend on the release of hydrogen peroxide, as it was prevented by catalase. However, when presented trypanosomes in presence of fresh immune serum which was itself lytic, macrophages did not show any additional lytic effect, and phagocytosis was related to already damaged parasites.
| Original language | English |
|---|---|
| Pages (from-to) | 131-140 |
| Number of pages | 10 |
| Journal | Experimental Parasitology |
| Volume | 65 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 1988 |
| Externally published | Yes |
Keywords
- Macrophage cytotoxicity
- Mouse macrophages
- Oxygen metabolites
- Parasitic protozoa
- Procyclic forms
- Trypanosoma brucei brucei