TY - JOUR
T1 - Tuning a parallel segmented flow column and enabling multiplexed detection
AU - Pravadali-Cekic, Sercan
AU - Kocic, Danijela
AU - Hua, Stanley
AU - Jones, Andrew
AU - Dennis, Gary R.
AU - Shalliker, R. Andrew
PY - 2015
Y1 - 2015
N2 - Active flow technology (AFT) is new form of column technology that was designed to overcome flow heterogeneity to increase separation performance in terms of efficiency and sensitivity and to enable multiplexed detection. This form of AFT uses a parallel segmented flow (PSF) column. A PSF column outlet end-fitting consists of 2 or 4 ports, which can be multiplexed to connect up to 4 detectors. The PSF column not only allows a platform for multiplexed detection but also the combination of both destructive and non-destructive detectors, without additional dead volume tubing, simultaneously. The amount of flow through each port can also be adjusted through pressure management to suit the requirements of a specific detector(s). To achieve multiplexed detection using a PSF column there are a number of parameters which can be controlled to ensure optimal separation performance and quality of results; that is tube dimensions for each port, choice of port for each type of detector and flow adjustment. This protocol is intended to show how to use and tune a PSF column functioning in a multiplexed mode of detection.
AB - Active flow technology (AFT) is new form of column technology that was designed to overcome flow heterogeneity to increase separation performance in terms of efficiency and sensitivity and to enable multiplexed detection. This form of AFT uses a parallel segmented flow (PSF) column. A PSF column outlet end-fitting consists of 2 or 4 ports, which can be multiplexed to connect up to 4 detectors. The PSF column not only allows a platform for multiplexed detection but also the combination of both destructive and non-destructive detectors, without additional dead volume tubing, simultaneously. The amount of flow through each port can also be adjusted through pressure management to suit the requirements of a specific detector(s). To achieve multiplexed detection using a PSF column there are a number of parameters which can be controlled to ensure optimal separation performance and quality of results; that is tube dimensions for each port, choice of port for each type of detector and flow adjustment. This protocol is intended to show how to use and tune a PSF column functioning in a multiplexed mode of detection.
KW - active flow technology
KW - liquid chromatography
KW - separation (technology)
UR - http://handle.uws.edu.au:8081/1959.7/uws:33891
U2 - 10.3791/53448
DO - 10.3791/53448
M3 - Article
SN - 1940-087X
VL - 106
SP - 1
EP - 10
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
M1 - e53448
ER -