TY - JOUR
T1 - Two dimensional reversed-phase reversed-phase separations : isomeric separations incorporating C18 and carbon clad zirconia stationary phases
AU - Gray, Michael J.
AU - Dennis, Gary R.
AU - Wormell, Paul
AU - Shalliker, Ross A.
AU - Slonecker, Patrick J.
PY - 2002
Y1 - 2002
N2 - Informational theory and a geometric approach to factor analysis were employed to evaluate the degree of orthogonality of a two-dimensional reversed-phase-reversed-phase chromatographic system. The system incorporated a C
18 column as one dimension and a carbon clad zirconia column as the second dimension. In order to study the resolving power of this system, the separation of a sample matrix containing an artificial mix of 32 isomers (structural and diastereoisomers) was evaluated. Using this system, between 25 and 28 of the 32 isomers could be separated, depending on the mobile phase combinations - with resolution that could not possibly be achieved in a single one dimensional separation. The results from this study indicate that in order to fully evaluate the resolving power of a 2D system multiple methods of analysis are most appropriate. This becomes increasingly important when the sample contains components that are very closely related and the retention of solutes is clustered in one quadrant of the 2D space. Ultimately, the usefulness of the 2D separation is determined by the goals of analyst.
AB - Informational theory and a geometric approach to factor analysis were employed to evaluate the degree of orthogonality of a two-dimensional reversed-phase-reversed-phase chromatographic system. The system incorporated a C
18 column as one dimension and a carbon clad zirconia column as the second dimension. In order to study the resolving power of this system, the separation of a sample matrix containing an artificial mix of 32 isomers (structural and diastereoisomers) was evaluated. Using this system, between 25 and 28 of the 32 isomers could be separated, depending on the mobile phase combinations - with resolution that could not possibly be achieved in a single one dimensional separation. The results from this study indicate that in order to fully evaluate the resolving power of a 2D system multiple methods of analysis are most appropriate. This becomes increasingly important when the sample contains components that are very closely related and the retention of solutes is clustered in one quadrant of the 2D space. Ultimately, the usefulness of the 2D separation is determined by the goals of analyst.
KW - Factor analysis
KW - High performance liquid chromatography
KW - Information theory
KW - Separation (Technology)
KW - Carbon clad zirconia
KW - Silica, octadecyl bonded
KW - Two-dimensional reversed-phase chromatography
KW - Stationary phases, LC
UR - http://handle.uws.edu.au:8081/1959.7/10550
UR - http://www.scopus.com/inward/record.url?scp=0036837346&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(02)01221-9
DO - 10.1016/S0021-9673(02)01221-9
M3 - Article
SN - 0021-9673
VL - 975
SP - 285
EP - 297
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -