Two dimensional reversed-phase reversed-phase separations : isomeric separations incorporating C18 and carbon clad zirconia stationary phases

Michael J. Gray, Gary R. Dennis, Paul Wormell, Ross A. Shalliker, Patrick J. Slonecker

    Research output: Contribution to journalArticle

    75 Citations (Scopus)

    Abstract

    Informational theory and a geometric approach to factor analysis were employed to evaluate the degree of orthogonality of a two-dimensional reversed-phase-reversed-phase chromatographic system. The system incorporated a C 18 column as one dimension and a carbon clad zirconia column as the second dimension. In order to study the resolving power of this system, the separation of a sample matrix containing an artificial mix of 32 isomers (structural and diastereoisomers) was evaluated. Using this system, between 25 and 28 of the 32 isomers could be separated, depending on the mobile phase combinations - with resolution that could not possibly be achieved in a single one dimensional separation. The results from this study indicate that in order to fully evaluate the resolving power of a 2D system multiple methods of analysis are most appropriate. This becomes increasingly important when the sample contains components that are very closely related and the retention of solutes is clustered in one quadrant of the 2D space. Ultimately, the usefulness of the 2D separation is determined by the goals of analyst.

    Original languageEnglish
    Pages (from-to)285-297
    Number of pages13
    JournalJournal of Chromatography A
    Volume975
    Issue number2
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Factor analysis
    • High performance liquid chromatography
    • Information theory
    • Separation (Technology)
    • Carbon clad zirconia
    • Silica, octadecyl bonded
    • Two-dimensional reversed-phase chromatography
    • Stationary phases, LC

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