TY - JOUR
T1 - Understanding and improving direct UV detection of monosaccharides and disaccharides in free solution capillary electrophoresis
AU - Oliver, James D.
AU - Rosser, Adam A.
AU - Fellows, Christopher M.
AU - Guillaneuf, Yohann
AU - Clement, Jean-Louis
AU - Gaborieau, Marianne
AU - Castignolles, Patrice
PY - 2014
Y1 - 2014
N2 - tDirect UV detection of carbohydrates in free solution capillary electrophoresis at 270 nm is made possibleby a photo-oxidation reaction. Glucose, rhamnose and xylose were shown to have unique UV absorptionspectra hypothesizing different UV absorbing intermediates for their respective photo-oxidation. NMRspectroscopy of the photo-oxidation end products proved they consisted of carboxylates and not malon-dialdehyde as previously theorized and that oxygen thus plays a key role in the photo-oxidation pathway.Adding the photo-initiator Irgacure®2959 in the background electrolyte increased sensitivity by 40% atan optimum concentration of 1 à 10−4mM and 1 à 10−8mM for conventional 50 μm i.d. capillaries andfor the corresponding extended light path capillaries, respectively.
AB - tDirect UV detection of carbohydrates in free solution capillary electrophoresis at 270 nm is made possibleby a photo-oxidation reaction. Glucose, rhamnose and xylose were shown to have unique UV absorptionspectra hypothesizing different UV absorbing intermediates for their respective photo-oxidation. NMRspectroscopy of the photo-oxidation end products proved they consisted of carboxylates and not malon-dialdehyde as previously theorized and that oxygen thus plays a key role in the photo-oxidation pathway.Adding the photo-initiator Irgacure®2959 in the background electrolyte increased sensitivity by 40% atan optimum concentration of 1 à 10−4mM and 1 à 10−8mM for conventional 50 μm i.d. capillaries andfor the corresponding extended light path capillaries, respectively.
UR - http://handle.uws.edu.au:8081/1959.7/537154
U2 - 10.1016/j.aca.2013.12.001
DO - 10.1016/j.aca.2013.12.001
M3 - Article
VL - 809
SP - 183
EP - 193
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -